Main Title |
Comparison of Extraction Methods for the Isolation of Lipids and PCBs from Mussel Homogenate. |
Author |
Latimer, J. S. ;
Quinn., J. G. ;
|
CORP Author |
Rhode Island Univ., Narragansett. Graduate School of Oceanography.;Environmental Research Lab., Narragansett, RI. |
Publisher |
Dec 90 |
Year Published |
1990 |
Report Number |
EPA/600/3-90/092 ; ERLN-X175 |
Stock Number |
PB91-127787 |
Additional Subjects |
Mussels ;
Lipids ;
Graphs(Charts) ;
Tables(Data) ;
Chemical analysis ;
Thin layer chromatography ;
Extraction ;
Polychlorobiphenyl compounds ;
Environmental monitoring ;
Bioaccumulation
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB91-127787 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
48p |
Abstract |
A standard mussel homogenate was analyzed for total lipid mass, lipid class components, and PCBs using four different extraction procedures in an attempt to determine the most appropriate method for use in bioaccumulation studies of lipophilic organic pollutants. The four procedures included: chloroform/methanol/water (similar to the classical Bligh and Dyer method), hexane/acetonitrile/water, and hexane/methanol/water extractions at controlled v/v/v ratios; and a hexane/acetonitrile method requiring minimal solvent volume ratio control. TLC analysis of the lipids, alone, was insufficient to determine qualitative distinctions between the extraction procedures studied; however, Iatroscan(TM) technology was able to provide information on both the qualitative and quantitative distribution of lipids in the various extracts. |
Supplementary Notes |
Sponsored by Environmental Research Lab., Narragansett, RI. |
Category Codes |
68D; 57B; 99A |
NTIS Prices |
PC A03/MF A01 |
Primary Description |
600/05 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
103918325 |
Origin |
NTIS |
Type |
CAT |