Main Title |
Tumor Promotion in Rat Liver. |
Author |
Herren, S. L. ;
Pereira, M. A. ;
|
CORP Author |
Health Effects Research Lab., Cincinnati, OH. |
Year Published |
1983 |
Report Number |
EPA/600/J-83/237; |
Stock Number |
PB84-245646 |
Additional Subjects |
Phenobarbital ;
Toxicology ;
Bioassay ;
Rats ;
Liver ;
Barbiturates ;
Reprints ;
Carcinogenesis
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB84-245646 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
9p |
Abstract |
An initiation promotion bioassay for chemical carcinogens and tumor promoters has been developed in rat liver using presumed preneoplastic lesions, foci of gamma-glutamyltranspeptidase (GGTase)-positive hepatocytes, as the endpoint. To evaluate the tumor-promoting activity of phenobarbital, rats were administered diethylnitrosamine (DENA), 2.0 mmole/kg, followed by 500 ppm phenobarbital in their drinking water. After 6 weeks of phenobarbital promotion, the rats, had an increased incidence of foci as compared to nonphenobarbital-treated rats. By 50 weeks, the number of foci in the nonpromoted animals equaled the number observed with promotion. The stability and progression of GGTase-positive foci was determined in rats that received a 2/3 partial hepatectomy, followed 24 hours later by DENA administration (0.3 mmole/kg). The rats then received 500 ppm phenobarbital in the drinking water for 7 weeks. After 7 weeks, half of the rats were continued on phenobarbital and the other half were removed from phenobarbital treatment. |
Supplementary Notes |
Pub. in Environmental Health Perspectives, v50 p123-129 1983. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Tumor Promotion in Rat Liver. |
PUB Date Free Form |
1983 |
Category Codes |
6T; 6E; 57Y; 57O |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
435627118 |
Origin |
NTIS |
Type |
CAT |