Main Title |
Genus- and Group-Specific Hybridization Probes for Determinative and Environmental Studies of Sulfate-Reducing Bacteria. |
Author |
Devereux, R. ;
Kane, M. D. ;
Winfrey, J. ;
Stahl, D. A. ;
|
CORP Author |
Illinois Univ. at Urbana-Champaign. Dept. of Veterinary Pathobiology.;Environmental Research Lab., Gulf Breeze, FL.;Office of Naval Research, Arlington, VA. |
Publisher |
c1992 |
Year Published |
1992 |
Report Number |
N00014-88-K-0093; EPA/600/J-93/064; |
Stock Number |
PB93-168987 |
Additional Subjects |
Sulfate-reducing bacteria ;
Nucleic acid hybridization ;
Oligonucleotide probes ;
Species diversity ;
Ribosomal RNA ;
Temperature ;
Binding sites ;
Phylogeny ;
Base sequence ;
Sequence alignment ;
Reprints ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB93-168987 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
10p |
Abstract |
A set of six oligonucleotides, complementary to conserved tracts of 16S rRNA from phylogenetically-defined groups of sulfate-reducing bacteria, was characterized for use as hybridization probes in determinative and environmental microbiology. Four probes were genus specific and identified Desulfobacterium spp., Desulfobacter spp., Desulfobulbus spp., or Desulfovibrio spp. The other two probes encompassed more diverse assemblages. One probe was specific for the phylogenetic lineage composed of Desulfococcus multivorans, Desulfosarcina variabilis, and Desulfobotulus sapovorans. The remaining probe was specific for Desulfobacterium spp., Desulfobacter spp., D. multivorans, D. variabilis, and D. sapovorans. Temperature of dissociation was determined for each probe and the designed specificities of each were evaluated by hybridizations against closely related nontargeted species. In addition, each probe was screened by using a 'phylogrid' membrane which consisted of nucleic acids from sixtyfour non-targeted organisms representing a diverse collection of eukarya, archaea, and bacteria. The value of these probes to studies in environmental microbiology was evaluated by hybridizations to 16S rRNAs of sulfate-reducing bacteria present in marine sediments. |
Supplementary Notes |
Pub. in Systematic and Applied Microbiology 15, p601-609 1992. Sponsored by Environmental Research Lab., Gulf Breeze, FL., and Office of Naval Research, Arlington, VA. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Genus- and Group-Specific Hybridization Probes for Determinative and Environmental Studies of Sulfate-Reducing Bacteria. |
Category Codes |
57K; 57F |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/04 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
314124337 |
Origin |
NTIS |
Type |
CAT |