Main Title |
Discussion Summary: Application of Biotechnology to the Systematics and Monitoring of Biocontrol Agents. |
Author |
Kawanishi, C. Y. ;
Hemming, B. C. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;Monsanto Agricultural Co., St. Louis, MO. Technology Div. |
Publisher |
1989 |
Year Published |
1989 |
Report Number |
EPA/600/D-89/149; |
Stock Number |
PB90-112764 |
Additional Subjects |
Deoxyribonucleic acids ;
Bacteria ;
Enterobacteriaceae ;
Biotechnology ;
Gene amplification ;
Ribosomal RNA
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB90-112764 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
7p |
Abstract |
The report is a general discussion of the application of biotechnology to the systematics and monitoring of microbial agents used in biotechnology. Present conventional methods such as microscopy, selective media, marker utilization, etc., were described. Two newer methods, rRNA sequencing and DNA amplification techniques were described and their usefulness in identifying and monitoring microbes used in biotechnology discussed. These were concluded to be the most useful new techniques. Automated methods for bacteria identification and quantification were discussed. These methods were shown to be in need of adaptation to agricultural agents. The relevance of these methodologies is to the Agency's regulation of biotechnology products. |
Supplementary Notes |
Prepared in cooperation with Monsanto Agricultural Co., St. Louis, MO. Technology Div. |
NTIS Title Notes |
Symposium paper. |
Category Codes |
57K; 57F |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/10 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
935219905 |
Origin |
NTIS |
Type |
CAT |