Main Title |
Metabolism of 1-Nitropyrene by Human, Rat, and Mouse Intestinal Flora: Mutagenicity of Isolated Metabolites by Direct Analysis of HPLC Fractions with a Microsuspension Reverse Mutation Assay. |
Author |
King, L. C. ;
Kohan, M. J. ;
George, S. E. ;
Lewtas, J. ;
Claxton, L. D. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. |
Publisher |
c1990 |
Year Published |
1990 |
Report Number |
EPA/600/J-90/423; |
Stock Number |
PB91-171785 |
Additional Subjects |
Metabolism ;
Enterobacteriaceae ;
Toxicology ;
High pressure liquid chromatography ;
Mutagenicity tests ;
Aromatic polycyclic hydrocarbons ;
Rats ;
Human ;
Mice ;
Reprints ;
1-Nitropyrene
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB91-171785 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
16p |
Abstract |
Among the nitro-substituted polycyclic aromatic hydrocarbons identified in environmental samples and known to be genotoxic, 1-nitropyrene is one of the most abundant. The biotransformation of 1-nitro((14)C)pyrene by human, rat, and mouse intestinal microflora and the mutagenicity of the isolated metabolites by direct analysis of the HPLC fractions with a microsuspension mutation assay were investigated. 1-nitro((14)C)pyrene was metabolized by human, rat and mouse intestinal microflora to the following reductive metabolites; 1-aminopyrene, Nacetylaminopyrene, N-formyl-1-aminopyrene and two unknown metabolites identified as A and B. The predominant metabolite of 1-nitro((14)C)pyrene produced by human, rat or mouse intestinal microflora following a 12 h incubation was 1-aminopyrene which accounted for 79 to 93% of the total (14)C respectively. Only minor amounts of N-formyl-1-aminopyrene (1%), N-acetylaminopyrene (3 - 4%) were produced. The similarity in the distribution of the reductive metabolites suggests that a similar mechanism exists in the biotransformation of 1-nitropyrene by intestinal microflora of different mammalian species. |
Supplementary Notes |
Pub. in Jnl. of Toxicology and Environmental Health, v31 n3 p179-192 Nov 90. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Metabolism of 1-Nitropyrene by Human, Rat, and Mouse Intestinal Flora: Mutagenicity of Isolated Metabolites by Direct Analysis of HPLC Fractions with a Microsuspension Reverse Mutation Assay. |
Category Codes |
57Y; 57F; 57B; 57K; 68G |
NTIS Prices |
PC A03/MF A01 |
Primary Description |
600/10 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
114324829 |
Origin |
NTIS |
Type |
CAT |