||Application of DNA Probes to Analysis of Bacteriophage Distribution Patterns in the Environment.
Ogunseitan, O. A. ;
Sayler, G. S. ;
Miller, R. V. ;
||Environmental Research Lab., Gulf Breeze, FL. ;Tennessee Univ., Knoxville. ;Oklahoma State Univ., Stillwater.
DNA probes ;
Aquatic microbiology ;
Soil microbiology ;
Pseudomonas aeruginosa ;
Viral genes ;
Genetic transduction ;
Nucleic acid hybridization ;
Agar gel electrophoresis ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||Radiolabeled bacteriophage DNA probes have been used in the study to determine the distribution of Pseudomonas aeruginosa-infecting bacteriophages in natural samples of lake water, sediment, soil, and sewage. The sensitivity of detection of bacteriophage with the DNA probes was between 100 and 1,000 PFU and ten to the sixth power to ten to the seventh power CFU of lysogenized bacteria detectable with a homologous phage DNA probe. Analyses of environmental samples suggest that up to 40% of P. aeruginosa in natural ecosystems contain DNA sequences homologous to phage genomes. By using different bacteriophage DNA probes, the diversity of the bacteriophage population in sewage was estimated to be higher than that in other natural samples. The indication that transducing phages and prophages are widely distributed in the Pseudomonas populations investigated has considerable implications for the frequency of natural gene transfer by transduction and of lysogenic conversion of host bacteria in natural ecosystems. (Copyright (c) 1992, American Society for Microbiology.)
||Pub. in Application and Environment Microbiology, v58 n6 p2046-2052 Jun 92. Prepared in cooperation with Tennessee Univ., Knoxville, and Oklahoma State Univ., Stillwater.
||Reprint: Application of DNA Probes to Analysis of Bacteriophage Distribution Patterns in the Environment.
||PC A02/MF A01