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OLS Field Name OLS Field Data
Main Title In vitro Bioassays for Toxic Polychlorinated Azobenzenes.
Author Sundstrom, G. ; Sawyer, T. ; Hutzinger, O. ; Safe, S. ;
CORP Author National Swedish Environment Protection Board, Solna. ;Texas A and M Univ., College Station. ;Bayreuth Univ. (Germany, F.R.). Chair of Ecological Chemistry and Geochemistry.;Environmental Research Lab.-Duluth, MN.
Publisher c1986
Year Published 1986
Report Number EPA-810995; EPA/600/J-86/540;
Stock Number PB90-265067
Additional Subjects Toxicity ; Chemical analysis ; Chlorinated hydrocarbons ; Anilines ; Bioassay ; In vitro analysis ; Gas chromatography ; Reprints ; Polychlorobiphenyl compounds
Holdings
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Status
NTIS  PB90-265067 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 12/03/1990
Collation 6p
Abstract Previous studies have demonstrated that the in vitro aryl hydrocarbon hydroxylase (AHH) and cytosolic receptor binding bioassays can be utilized to quantitatively estimate the potential in vivo toxicities of polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs) and their mixtures (from municipal fly ash extracts). The study reports the use of the two bioassays in estimating the levels of 3,3',4,4'-tetrachloroazobenzene in several commercial products which contain or are derived from 3,4-dichloroaniline. The AHH induction and receptor binding EC50 values for 3,3',4,4'-tetrachloroazobenzene were 5.60 X 10 to the minus seventh and 5.60 X 10 to the minus sixth M respectively. The levels of 3,3',4,4'-tetrachloroazobenzene (TCAB) in commercial samples of Diuron, Linuron and 3,4-dichloroaniline were determined by gas chromatographic (GC) analysis and the concentrations varied from trace levels to 2200 ug/g. Preliminary bioassays of the commercial compounds and crude extracts (BC) of these products gave contradictory results which did not correlate with the GC analytical data. Presumably there are impurities or coextractives which interfere with the sensitivity of the bioassays. The crude extracts were purified by column chromatography and were assayed using both bioassays.
Supplementary Notes Pub. in Chemosphere, v15 n9-12 p2105-2107 1986. Portions of this document are not fully legible. Prepared in cooperation with Texas A and M Univ., College Station, and Bayreuth Univ. (Germany, F.R.). Chair of Ecological Chemistry and Geochemistry. Sponsored by Environmental Research Lab.-Duluth, MN.
NTIS Title Notes Journal article.
Title Annotations Reprint: In vitro Bioassays for Toxic Polychlorinated Azobenzenes.
Category Codes 99A; 57Y
NTIS Prices PC A02/MF A01
Primary Description 600/03
Document Type NT
Cataloging Source NTIS/MT
Control Number 028533285
Origin NTIS
Type CAT