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RECORD NUMBER: 3 OF 964

OLS Field Name OLS Field Data
Main Title (32)P-Postlabeling Analysis of DNA Adducts of Two Nitrated Polycyclic Aromatic Hydrocarbons in Rabbit Tracheal Epithelial Cells.
Author Gallagher, J. E. ; Robertson, I. G. C. ; Jackson, M. A. ; Dietrich, A. M. ; Ball, L. M. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.;North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering.
Year Published 1987
Report Number EPA/600/D-87/240;
Stock Number PB88-103718
Additional Subjects Aromatic polycyclic hydrocarbons ; Deoxyribonucleic acids ; Cells(Biology) ; Epithelium ; Trachea ; Rabbits ; Marking ;
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB88-103718 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 9p
Abstract The 1-nitropyrene (1-NPP and 3-nitrofluoranthene (3-NF) adducts have been analyzed by (32)P-postlabeling and with 1-NP have been compared to the total number of adducts estimated from (14)C binding in rabbit trachael epithelial (RTE) DNA samples. One adduct spot, by (32)P-postlabeling, was detected in the DNA of RTE cells treated with (14)C-1-NP. The adduct, at approximately 6 adducts 10-to the 9th power nucleotides, represents a minor component of the binding to RTE DNA being lesser than 1% of the total (14)C-radioactivity associated with the extracted DNA. Treatment of RTE cells with unlabeled 3-NF resulted in two adduct spots at approximately 12 adducts/10 to the 9th power. When the P1 nuclease treatment was used as an alternative method both the 3-NF derived adducts previously detected following the butanol extraction were absent.
Supplementary Notes Prepared in cooperation with Environmental Health Research and Testing, Inc., Research Triangle Park, NC. Sponsored by North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering.
PUB Date Free Form Sep 87
Category Codes 57F
NTIS Prices PC A02/MF A01
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 733831751
Origin NTIS
Type CAT