Main Title |
Determination of Plasmid DNA Concentration Maintained by Nonculturable 'Escherichia coli' in Marine Microcosms. |
Author |
Byrd, J. J. ;
Leahy, J. G. ;
Colwell, R. R. ;
|
CORP Author |
Center of Marine Biotechnology, Baltimore, MD.;National Science Foundation, Washington, DC.;Environmental Research Lab., Gulf Breeze, FL. |
Publisher |
c1992 |
Year Published |
1992 |
Report Number |
NSF-BSR-8806509; EPA/600/J-92/386; |
Stock Number |
PB93-121176 |
Additional Subjects |
Aquatic microbiology ;
Escherichia coli ;
Plasmids ;
Deoxyribonucleic acids ;
Marine biology ;
Cell survival ;
Molecular cloning ;
Sea water ;
Microbial colony count ;
Reprints ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB93-121176 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
7p |
Abstract |
The concentration of plasmid pBR322 DNA in nonculturable Escherichia coli JM83 was measured to determine whether the plasmid concentration changed during survival of E. coli in marine and estuarine water. E. coli JM83 containing the plasmid pBR322 was placed in both sterile seawater and sterile estuarine water and analyzed for survival (i.e., culturability) and plasmid maintenance. The concentration of pBR322 DNA remained stable in E. coli JM83 for 28 days in an artificial seawater microcosm, even though nonculturability was achieved within 7 days. E. coli JM83 incubated in sterile natural seawater or sterile estuarine water did not reach nonculturability within 30 days. Under all three conditions, plasmid pBR322 DNA was maintained at approximately the initial concentration. Cloning of DNA into the plasmid pUC8 did not alter the ability of E. coli to maintain vector plasmid DNA, even when the culture was in the nonculturable state, but the concentration of plasmid DNA decreased with time in the microcosm. The authors conclude that E. coli is able to maintain plasmid DNA while in the nonculturable state and that the concentration at which the plasmid is maintained appears to be dependent upon the copy number of the plasmid and/or the presence of foreign DNA. (Copyright (c) 1992, American Society for Microbiology.) |
Supplementary Notes |
Pub. in Applied and Environmental Microbiology, v58 n7 p2266-2270 Jul 92. Sponsored by National Science Foundation, Washington, DC., and Environmental Research Lab., Gulf Breeze, FL. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Determination of Plasmid DNA Concentration Maintained by Nonculturable 'Escherichia coli' in Marine Microcosms. |
Category Codes |
57K; 57F; 47D |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/04 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
300826275 |
Origin |
NTIS |
Type |
CAT |