||Direct viable counting method for meauring tolerance of aquatic microbial communities to Hg(2+1) /
Liebert, Cynthia. ;
||Environmental Research Lab., Gulf Breeze, FL. ;Technology Applications, Inc., Gulf Breeze, FL.
|| U.S. Environmental Protection Agency, Office of Research and Development, Environmental Research Laboratory,
Aquatic microbiology ;
Toxic tolerances ;
Escherichia coli ;
Cell division ;
Acid treatment ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||8 pages : illustrations ; 28 cm
||Direct counts of Hg2+ resistant cells in natural waters were obtained by fluorescence microscopy after incubation for 20 hours in the presence of a growth substrate, nalidixic acid to halt cell division, and inhibiting concentrations of Hg2+. The method discriminated Hg2+ resistant from sensitive Escherichia coli strains. Estuarine samples were used to compare this procedure with two other toxicity measurements that determine the effect of Hg2+ on colony growth and on rates of radioactive thymidine incorporation into cellular material. Toxicity measurements based on direct viable counts and thymidine incorporation rates had comparable sensitivities, and both were 3-4 orders of magnitude more sensitive than the method that utilized colony counts. Thus, the direct enumeration of Hg2+ resistant cells is useful for predicting the potential of aquatic communities to sustain heterotrophic activity, an essential microbial process, in the presence of Hg2+.
||"Reprint article published in Canadian Journal of Microbiology, 34:1090-1095 1988." Caption title. "EPA/600/J-88/351." Microfiche.
||Gulf Breeze, FL :
||Pub in the Canadian Jnl. of Microbiology, v34 p1090-1095 1988. Prepared in cooperation with Technology Applications, Inc., Gulf Breeze, FL.
|Corporate Au Added Ent
||United States. Environmental Protection Agency. Office of Research and Development. ; Environmental Research Laboratory.
|PUB Date Free Form
||PC A02/MF A01
|OCLC Time Stamp
|OCLC Rec Leader
||01203nam 2200289Ka 45020