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OLS Field Name OLS Field Data
Main Title DNA Sequence Analysis of Revertants of the 'hisD3052' Allele of 'Salmonella typhimurium' TA98 Using the Polymerase Chain Reaction and Direct Sequencing: Application to 1-Nitropyrene-Induced Revertants.
Author Bell, D. A. ; Levine, J. G. ; DeMarini., D. M. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-91/098;
Stock Number PB91-200329
Additional Subjects Bacterial DNA ; Salmonella typhimurium ; Mutation ; Polymerase chain reaction ; Alleles ; Base sequence ; Metabolic activation ; Single-stranded DNA ; Nucleic acid conformation ; Agar gel electrophoresis ; Reprints ; 1-Nitropyrene
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NTIS  PB91-200329 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 09/04/1991
Collation 12p
Abstract The study used the polymerase chain reaction (PCR) to speed the processing of revertants of Salmonella typhimurium TA98 for DNA sequence analysis. Briefly, a crude DNA extract from a single colony was prepared and used in an asymmetric PCR to amplify a 228-bp fragment containing the hisD3052 mutation approximately in the center. Following ultra-filtration, the ssDNA was sequenced using an end-labeled probe and dideoxy sequencing. The most frequent mutation among the revertants was a -2 deletion of GC or CG within the sequence CGCGCGCG, which is upstream of the hisD3052 mutation. The deletion occurred in 38% (6/16) of the spontaneous (-S9) revertants and in 94% (15/16) of a set of 1-nitropyrene-induced revertants. Misalignment of complementary DNA strands within this repeat may account for this mutation, although the possible formation of Z-DNA within this region may also play a role. Other mutations, mostly deletions but also some complex mutations (insertions/deletions/substitutions), occurred within quasi-palindromic regions of DNA. The potential DNA secondary structures within such regions may mediate the templated production of some of these mutations.
Supplementary Notes Pub. in Mutation Research, v252 n1 p35-44 Feb 91.
NTIS Title Notes Journal article.
Title Annotations Reprint: DNA Sequence Analysis of Revertants of the 'hisD3052' Allele of 'Salmonella typhimurium' TA98 Using the Polymerase Chain Reaction and Direct Sequencing: Application to 1-Nitropyrene-Induced Revertants.
Category Codes 57K; 57F
NTIS Prices PC A03/MF A01
Primary Description 600/10
Document Type NT
Cataloging Source NTIS/MT
Control Number 122131013
Origin NTIS
Type CAT