||Cytotoxicity and Effect on Mutagenicity of Buffers in a Microsuspension Assay.
DeMarini, D. M. ;
Dallas, M. M. ;
Lewtas., J. ;
||Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Metabolic activation ;
Salmonella typhimuriUm ;
Exhaust gases ;
Cell survival ;
Mutagenicity tests ;
Tobacco smoke pollution ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||In the presence of S9, the mutagenic potency of 2-aminoanthracene was similar among the three buffers; likewise, the mutagenic potency of environmental tobacco smoke was also similar among the buffers. In the absence of S9, the 0.15 M buffer resulted in reduced mutagenic potencies of 1-nitropyrene and disel exhaust compared to the respective potencies of these agents with the other two buffers. The cell concentrations had little effect on the mutagenic potencies of these agents with or without S9. In the absence of S9, the 0.15 M buffer was highly cytotoxic, resulting in 23% survival of the control cells after 90 min of incubation, compared to 89 and 35% survival, respectively, for 0.015 M and VBM. In the presence of S9, the 0.15 M buffer resulted in 57% survival, compared to 294 and 80% survival, respectively, for 0.015 M and VBM.
||Pub. in Teratogenesis, Carcinogenesis, and Mutagenesis, v9 n5 p287-295 Oct 89. Prepared in cooperation with Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
|NTIS Title Notes
||Reprint: Cytotoxicity and Effect on Mutagenicity of Buffers in a Microsuspension Assay.
||57Y; 57F; 68A
||PC A03/MF A01