Main Title |
Effect of Incubation and Activation Conditions on the Hepatocyte-Mediated Plate Incorporation and Preincubation 'Salmonella typhimurium' Mutagenesis Assays. |
Author |
Williams, K. ;
Inmon, J. ;
Lewtas, J. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. |
Year Published |
1983 |
Report Number |
EPA-600/J-83-137; |
Stock Number |
PB84-144336 |
Additional Subjects |
Bacteria ;
Reprints ;
Salmonella typhimurium ;
Mutagenesis ;
Cell mediated assays
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB84-144336 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
12p |
Abstract |
Primary cell-mediated microbial mutagenesis assays have been shown to be useful in detecting specific target organ genotoxic activity. The lack of a standard protocol for these assays, however, makes interlaboratory comparisons difficult. In order to standardize the hepatocyte-mediated Salmonella typhimurium mutagenesis assay, incubation and activation conditions for the plate incorporation and preincubation assays were examined using two aromatic amines, 2-aminofluorene (AF) and 2-acetylaminofluorene (AAF). Direct comparison of two preincubation protocols demonstrated the necessity for the hepatocytes to be present during the two-to three-day plate incubation period. |
Supplementary Notes |
Pub. in Teratogenesis, Carcinogenesis, and Mutagenesis 3, p367-376 1983. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Effect of Incubation and Activation Conditions on the Hepatocyte-Mediated Plate Incorporation and Preincubation 'Salmonella typhimurium' Mutagenesis Assays. |
PUB Date Free Form |
1983 |
Category Codes |
6M; 57K |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
409019538 |
Origin |
NTIS |
Type |
CAT |