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RECORD NUMBER: 47 OF 74

OLS Field Name OLS Field Data
Main Title Physicochemical Interaction of 'Escherichia coli' Cell Envelopes and 'Bacillus subtilis' Cell Walls with Two Clays and Ability of the Composite to Immobilize Heavy Metals from Solution.
Author Walker, S. G. ; Flemming, C. A. ; Ferris, F. G. ; Beveridge, T. J. ; Bailey, G. W. ;
CORP Author Guelph Univ. (Ontario).;Environmental Research Lab., Athens, GA.
Publisher c1989
Year Published 1989
Report Number EPA-R-813605; EPA/600/J-89/251;
Stock Number PB90-135500
Additional Subjects Adsorption ; Bacillus subtilis ; Escherichia coli ; Clay minerals ; Chemical reactions ; Cell wall ; Kaolinite ; Centrifuging ; Metals ; Cation exchanging ; Binding ; Waste disposal ; X-ray spectroscopy ; Reprints ; Heavy metals ; Smectite
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB90-135500 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/15/1990
Collation 11p
Abstract Isolated Escherichia coli K-12 cell envelopes or Bacillus subtilis 168 cell walls were reacted with smectite or kaolinite clay in distilled deionized water (pH 6.0); unbound envelopes or walls were separated by sucrose density gradient centrifugation, and the extent of adsorption was calculated. At saturation, both clays adsorbed approximately 1.0 mg (dry weight) of envelopes or walls per mg (dry weight) of clay. The addition of heavy metals increased the incidence of planar surface orientations, which suggested that multivalent metal cation bridging was coming into play and was of increasing importance. On a dry-weight basis, the envelope-clay and wall-clay mixtures bound 20 to 90% less metal than equal amounts of the individual components did. This reduction in metal-binding capacity indicates that the adsorption of the wall or envelope to clay has masked or neutralized chemically reactive adsorption sites normally available to metal ions. Transmission electron microscopy and energy-dispersive X-ray spectroscopy confirmed these results.
Supplementary Notes Pub. in Applied and Environmental Microbiology, v55 n11 p2976-2984 Nov 89. Sponsored by Environmental Research Lab., Athens, GA.
NTIS Title Notes Journal article.
Title Annotations Reprint: Physicochemical Interaction of 'Escherichia coli' Cell Envelopes and 'Bacillus subtilis' Cell Walls with Two Clays and Ability of the Composite to Immobilize Heavy Metals from Solution.
Category Codes 57K; 57B; 68D; 48F
NTIS Prices PC A03/MF A01
Primary Description 600/01
Document Type NT
Cataloging Source NTIS/MT
Control Number 005317659
Origin NTIS
Type CAT