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OLS Field Name OLS Field Data
Main Title Stress Ethylene: A Bioassay for Rhizosphere-Applied Phytotoxicants.
Author Rodecap, Kent D. ; Tingey, David T. ;
CORP Author Corvallis Environmental Research Lab., OR.
Year Published 1981
Report Number EPA-600/J-81-523;
Stock Number PB82-169186
Additional Subjects Ethylene ; Toxicology ; Plants(Botany) ; Bioassay ; Responses ; Stems(Botany) ; Reprints ; Phytotoxicants
Library Call Number Additional Info Location Last
NTIS  PB82-169186 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation 11p
Abstract A bioassay for rhizosphere-applied phytotoxicants was developed and evaluated with a broad range of chemicals. Test substances were applied to the rhizosphere of whole, intact bush bean plants (Phaseolus vulgaris L. cv. Bush Blue Lake 290) grown in a solid support medium and the resultant ethylene production was measured to detect the presence of phytotoxic materials. The beans were encapsulated in plastic bags for 2 hrs following treatment and then incubated for 24 hrs in the dark. Ethylene and ethane accumulating within the bags were quantified via gas-solid chromatography. The application of various concentration of inorganic and organic chemicals induced various responses. No single equation adequately described the dose-response curves; therefore, a critical value (a statistically significant increase in stress ethylene production) was computed for each test substance. A phytotoxic-response threshold for each test substance was defined as the test substance concentration that caused ethylene production to exceed its respective critical-value concentration. Based on threshold concentrations determined by analysis of stress ethylene production, the relative phytotoxicity rankings of the inorganic test substances were: CdCl2 > CuCl2 > Pb(C2H302)2 > LiCl, while those of the organic test substances were 2,4-D esters < paraquat dicloride < 1-butanol < 2-propanone < 2-propanol. Both stress ethylene and ethane production were nonresponsive to hydrogen-ion concentration (measured before application) over a broad range.
Supplementary Notes Pub. in Environmental Monitoring and Assessment 1, p119-127 1981.
NTIS Title Notes Journal article.
Title Annotations Reprint: Stress Ethylene: A Bioassay for Rhizosphere-Applied Phytotoxicants.
PUB Date Free Form 1981
Category Codes 6T; 6C; 57Y; 57C
NTIS Prices PC A02/MF A01
Primary Description 600/02
Document Type NT
Cataloging Source NTIS/MT
Control Number 323811586
Origin NTIS
Type CAT