||Restriction Fragment Length Polymorphisms Distinguish Ectomycorrhizal Fungi.
Armstrong, J. L. ;
Fowles, N. L. ;
Rygiewicz, P. T. ;
||Corvallis Environmental Research Lab., OR. ;NSI Technology Services Corp., Corvallis, OR.
Plant genetics ;
Deoxyribonucleic acids ;
Restriction fragment length polymorphisms ;
Ectomycorrhizal fungi ;
Nucleic acid hybridization ;
DNA probes ;
Laccaria bicolor ;
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||Basidiomycetous fungi, two saprophytes and three mycorrhizal, were used to assess the specificity of DNA hybridization for distinguishing genera from one another. Interspecific comparisons were done with several isolates of mycorrhizal fungi, Laccaria bicolor and L. Laccata, collected from diverse geographical sites. The DNAs were digested with four restriction nucleases and separated by gel electrophoresis into patterns of DNA fragments called restriction fragment length polymorphism (RFLPs). The RFLPs were hybridized with a radioactively-labeled DNA probe encoding Basidiomycetous ribosomal RNA genes. The five genera were discernable using both unprobed and probed RFLPs. Hybridization of probe DNA with RFLPs was isolate-specific for all nine Laccaria isolates examined. The reclassification of a L. bicolor isolate is supported, demonstrating that hybridization of RFLPs offers an additional tool for taxonomy of ectomycorrhizal fungi. The method may have field application for distinguishing known isolates if their DNA fingerprints are previously ascertained and are distinct from RFLPs of indigenous organisms. (Copyright (c) Kluwer Academic Publishers 1989.)
||Pub. in Plant and Soil, v116 p1-7 1989. Prepared in cooperation with NSI Technology Services Corp., Corvallis, OR.
|NTIS Title Notes
||Reprint: Restriction Fragment Length Polymorphisms Distinguish Ectomycorrhizal Fungi.
||57C; 57F; 68G
||PC A02/MF A01