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OLS Field Name OLS Field Data
Main Title Restriction Fragment Length Polymorphisms Distinguish Ectomycorrhizal Fungi.
Author Armstrong, J. L. ; Fowles, N. L. ; Rygiewicz, P. T. ;
CORP Author Corvallis Environmental Research Lab., OR. ;NSI Technology Services Corp., Corvallis, OR.
Publisher c1989
Year Published 1989
Report Number EPA/600/J-89/112;
Stock Number PB90-108432
Additional Subjects Plant genetics ; Deoxyribonucleic acids ; Taxonomy ; Reprints ; Restriction fragment length polymorphisms ; Ectomycorrhizal fungi ; Nucleic acid hybridization ; DNA probes ; Laccaria bicolor ; Laccaria laccata
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NTIS  PB90-108432 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/10/1990
Collation 9p
Abstract Basidiomycetous fungi, two saprophytes and three mycorrhizal, were used to assess the specificity of DNA hybridization for distinguishing genera from one another. Interspecific comparisons were done with several isolates of mycorrhizal fungi, Laccaria bicolor and L. Laccata, collected from diverse geographical sites. The DNAs were digested with four restriction nucleases and separated by gel electrophoresis into patterns of DNA fragments called restriction fragment length polymorphism (RFLPs). The RFLPs were hybridized with a radioactively-labeled DNA probe encoding Basidiomycetous ribosomal RNA genes. The five genera were discernable using both unprobed and probed RFLPs. Hybridization of probe DNA with RFLPs was isolate-specific for all nine Laccaria isolates examined. The reclassification of a L. bicolor isolate is supported, demonstrating that hybridization of RFLPs offers an additional tool for taxonomy of ectomycorrhizal fungi. The method may have field application for distinguishing known isolates if their DNA fingerprints are previously ascertained and are distinct from RFLPs of indigenous organisms. (Copyright (c) Kluwer Academic Publishers 1989.)
Supplementary Notes Pub. in Plant and Soil, v116 p1-7 1989. Prepared in cooperation with NSI Technology Services Corp., Corvallis, OR.
NTIS Title Notes Journal article.
Title Annotations Reprint: Restriction Fragment Length Polymorphisms Distinguish Ectomycorrhizal Fungi.
Category Codes 57C; 57F; 68G
NTIS Prices PC A02/MF A01
Primary Description 600/02
Document Type NT
Cataloging Source NTIS/MT
Control Number 932621058
Origin NTIS
Type CAT