||Determination of Acrylamide in Rat Serum and Sciatic Nerve by Gas Chromatography-Electron-Capture Detection.
Raymer, J. H. ;
Sparacino, C. M. ;
Velez, G. R. ;
Padilla, S. ;
MacPhail, R. C. ;
||Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div. ;Research Triangle Inst., Research Triangle Park, NC. Analytical and Chemical Sciences Unit.
Sciatic nerve ;
Chemical analysis ;
Gas chromatography ;
Mass spectroscopy ;
Dose-response relationships ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||A modified method for the derivatization and determination of acrylamide as 2-bromopropenamide by gas chromatography-electron-capture detection was developed and applied to serum and sciatic nerve from rats. The method was accurate and precise over the calibration range 2.24-7.47 micrograms/ml in serum diluted 1:125 and 4-122 micrograms/g in sciatic nerve homogenate (5 mg/ml). Limits of detection were estimated to be 1,200 ng/ml in undiluted serum and 3 micrograms/g in intact sciatic nerve. The use of less dilute samples to allow for lower limits of detection appears feasible. The time-course of acrylamide in serum and sciatic nerve was studied after acute dosing and indicated elimination half-lives of 1.8 and 2.0 h for serum and sciatic nerve, respectively. A dose-effect relationship was established for each matrix after acute dosing and the measured acrylamide concentrations in serum (micrograms/ml) were approximately the same as in sciatic nerve (micrograms/g). (Copyright (c) 1993 Elsevier Science Publishers B.V.)
||Pub. in Jnl. of Chromatography 619, n2 p223-234 29 Oct 93. Prepared in cooperation with Research Triangle Inst., Research Triangle Park, NC. Analytical and Chemical Sciences Unit.
|NTIS Title Notes
||Reprint: Determination of Acrylamide in Rat Serum and Sciatic Nerve by Gas Chromatography-Electron-Capture Detection.
||PC A03/MF A01