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OLS Field Name OLS Field Data
Main Title Phenylacetic Acid Metabolism by Three Aquatic Bacteria Isolated from Continuous Culture Enrichments.
Author Mahaffey, W. R. ; Pritchard, P. H. ; Bourquin, A. W. ;
CORP Author Environmental Research Lab., Gulf Breeze, FL.
Year Published 1979
Report Number EPA-600/J-79-052 ;CONTRIB-377;
Stock Number PB80-163736
Additional Subjects Bacteria ; Organic compounds ; Cells(Biology) ; Cultures(Biology) ; Metabolism ; Microorganisms ; Concentration(Composition) ; Reprints ; Phenylacetic acid ; Toxic substances
Library Call Number Additional Info Location Last
NTIS  PB80-163736 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
Collation 8p
Abstract The fate of toxic organic compounds in aquatic environments depends, in part, on the capacity of microorganisms to metabolize these compounds at very low concn. Little information exists on the fate of aromatic ring compounds at low concn (<100 mg/l) in aquatic environments. Therefore, we attempt to characterize the degradation of the aromatic compound phenylacetic acid (PAA) at low concn by aquatic bacteria. Three bacterial isolates, PAL-1, PAL-10, and PAL-100 were obtained from continuous culture enrichment experiments at PAA concn of 1, 10, and 100 mg/liter, respectively. Washed cell suspensions of each isolate metabolized PAA, as measured by oxygen uptake, without lag regardless of whether cells were grown in acetate or PAA minimal salts medium, suggesting PAA metabolism was constitutive. Acetate-grown PAL-1 and PAL-100 cultures showed greater oxygen uptake activity in the presence of PAA than did PAA-grown cultures. All three isolates had high activity in the presence of meta-hydroxy-PAA. Incubation of washed cell suspensions at 25C for 12 h showed that only the PAL-1 isolate lost activity (75%) relative to cell suspensions incubated at 4C. The studies provide physiological criteria that may characterize the types of bacteria that transform aromatic compounds at low concn in aquatic environments.
Supplementary Notes Pub. in Journal of Development in Industrial Microbiology, v20, p489-495 1979.
NTIS Title Notes Journal article.
Title Annotations Reprint: Phenylacetic Acid Metabolism by Three Aquatic Bacteria Isolated from Continuous Culture Enrichments.
PUB Date Free Form 1979
Category Codes 6T; 6M; 8A; 57Y; 57K; 47D
NTIS Prices PC A02/MF A01
Primary Description 600/04
Document Type NT
Cataloging Source NTIS/MT
Control Number 323777169
Origin NTIS
Type CAT