Main Title |
Efficiency of Beef Extract for the Recovery of Poliovirus from Wastewater Effluents. |
Author |
Landry, Edward F. ;
Vaughn, James M. ;
Thomas, McHarrell Z. ;
Vicale, Thomas J. ;
|
CORP Author |
Brookhaven National Lab., Upton, NY.;Health Effects Research Lab., Cincinnati, OH. |
Year Published |
1978 |
Report Number |
EPA-R-804776-01; EPA-600/J-78-183; |
Stock Number |
PB81-213126 |
Additional Subjects |
Polioviruses ;
Waste water ;
Effluents ;
Efficiency ;
Viruses ;
Filters ;
Reprints ;
Beef extract ;
Sewage treatment effluents ;
NTISEPAORD
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB81-213126 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
7p |
Abstract |
The efficiency of poliovirus elution from fiber glass cartridge filters (K27), epoxy-fiber glass-asbestos filters (M780), and pleated cartridge filters was assessed by using 3% beef extract (pH 9.0) or 0.1 M glycine (pH 11.5). Poliovirus type I, strain LSc, was seeded into 20- to 25-gallon (ca. 75.6- to 95.6-liter) samples of treated sewage effluent and concentrated by using a filter adsorption-elution technique. Virus elution was accomplished by using either two 600-ml portions of 3% beef extract (pH 9.0), or two 1-liter portions of 0.1M glycine (pH 11.5). In all experiments, beef extract elution followed by organic flocculation was found to be superior, yielding a mean recovery efficiency of 85%, with recoveries ranging from 68 to 100%. Elution with 0.1 M glycine (pH 11.5) followed by inorganic flocculation resulted in a mean recovery efficiency of 36%. The variable range of recoveries with beef extract could not be significantly improved by varying the type of beef extract or by extending the elution time to 30 min. Second-step reconcentration of 1-liter seeded sewage effluent and renovated wastewater samples indicated that organic flocculation was a more efficient method for virus recovery than inorganic flocculation. Beef extract concentrations of less than 3% were found to be efficient in the recovery of poliovirus from renovated wastewater. |
Supplementary Notes |
Pub. in Applied and Environmental Microbiology, v36 n4 p544-548 Oct 78. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Efficiency of Beef Extract for the Recovery of Poliovirus from Wastewater Effluents. |
PUB Date Free Form |
26 Jul 78 |
Category Codes |
6M; 6F; 57K; 68D |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/10 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
323799756 |
Origin |
NTIS |
Type |
CAT |