Full Record Display for the EPA National Library Catalog

RECORD NUMBER: 10 OF 19

OLS Field Name OLS Field Data
Main Title Isolation and Characterization of the Alkane-Inducible NADPH-Cytochrome P-450 Oxidoreductase Gene from 'Candida tropicalis'.
Author Sutter, T. R. ; Sanglard, D. ; Loper, J. C. ;
CORP Author Cincinnati Univ., OH. Coll. of Medicine.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-93/327;
Stock Number PB93-229458
Additional Subjects Enzyme induction ; NADPH cytochrome reductase ; Alkanes ; Flavoproteins ; Messenger RNA ; Amino acid sequence ; Sequence alignment ; Reprints ; Candida tropicalis
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB93-229458 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 11/22/1993
Collation 12p
Abstract The gene coding for the Candida tropicalis NADPH-cytochrome P-450 oxidoreductase was isolated by immunoscreening of a C. tropicalis (lambda)gt11 expression library and colony hybridization of a C. tropicalis genomic library. The C. tropicalis CPR gene produces a 2.35-kilobase mRNA transcript, levels of which were shown to be increased 16-fold in cells grown on tetradecane relative to cells grown on glucose as the sole carbon source. A 3-kilobase DNA fragment was sequenced, including 554 and 397 base pairs of 5'- and 3'-noncoding sequence, respectively. A single open reading frame of 2040 base pairs was identified and predicts a 76,683-Da polypeptide of 680 amino acid residues. The deduced C. tropicalis CPR amino acid sequence was compared with each of the CPR sequences reported from other organisms and invariant residues were identified. (Copyright (c) 1990 by The American Society for Biochemistry and Molecular Biology, Inc.)
Supplementary Notes Pub. in Jnl. of Biological Chemistry, v265 n27, p16428-16436, 25 Sep 90. Sponsored by Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
NTIS Title Notes Journal article.
Title Annotations Reprint: Isolation and Characterization of the Alkane-Inducible NADPH-Cytochrome P-450 Oxidoreductase Gene from 'Candida tropicalis'.
Category Codes 57F; 57K
NTIS Prices PC A03/MF A01
Primary Description 600/14
Document Type NT
Cataloging Source NTIS/MT
Control Number 328023473
Origin NTIS
Type CAT