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OLS Field Name OLS Field Data
Main Title Preparation of Biological Tissue Sections for Correlative Ion, Electron, and Light Microscopy.
Author Kupke, K. G. ; Pickett, J. P. ; Ingram, P. ; Griffis, D. P. ; Linton, R. W. ;
CORP Author Duke Univ. Medical Center, Durham, NC. ;Veterans Administration Medical Center, Durham, NC. ;Research Triangle Inst., Research Triangle Park, NC. ;North Carolina Univ. at Chapel Hill. Dept. of Chemistry.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1984
Report Number EPA-R-807560; EPA/600/J-84/354;
Stock Number PB86-136223
Additional Subjects Electron microscopy ; Microscopy ; Tissues(Biology) ; Reprints ; Ion microscopy
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NTIS  PB86-136223 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 12p
Abstract In order to correctly interpret the chemical images obtained using ion microscopy (IM), it is useful to correlate them with the information provided by conventional light microscopy (LM), secondary electron imaging (SEI), backscattered electron imaging (BEI), and electron probe microanalysis (EPMA). Accordingly, the authors have devised a technique of specimen preparation which allows for the application of several different microanalytical techniques to a single histologic section mounted on the same substrate. Sections are cut onto polyester plastic coverslips (devoid of peaks for any element with atomic number >9 using EPMA) and studied by LM. After a light rotary coating with carbon (to prevent charging), the section can then be examined by SEI, BEI, and EPMA. Specific areas can be marked for IM study either with an objective-mounted pin tissue microlocater, or by placing small pieces of metal foil, cut in specific geometric shapes, over features of interest.
Supplementary Notes Prepared in cooperation with Veterans Administration Medical Center, Durham, NC., Research Triangle Inst., Research Triangle Park, NC., and North Carolina Univ. at Chapel Hill. Dept. of Chemistry. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
Availability Notes Pub. in Jnl. of Electron Microscopy Technique 1, p299-309 1984.
NTIS Title Notes Journal article.
Title Annotations Reprint: Preparation of Biological Tissue Sections for Correlative Ion, Electron, and Light Microscopy.
PUB Date Free Form c1984
Category Codes 6C; 57F
NTIS Prices Not available NTIS
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 607218368
Origin NTIS
Type CAT