Main Title |
Comparison of the Effects of Acute and Subacute Treatment of Phenobarbital in Different Strains of Mice. |
Author |
Lin, E. L. C. ;
Klaunig, J. E. ;
Mattox, J. K. ;
Weghorst, C. M. ;
McFarland, B. H. ;
|
CORP Author |
Health Effects Research Lab., Cincinnati, OH. ;Medical Coll. of Ohio at Toledo. ;Environmental Health Research and Testing, Inc., Cincinnati, OH. |
Publisher |
c1989 |
Year Published |
1989 |
Report Number |
EPA/600/J-89/332; |
Stock Number |
PB90-199415 |
Additional Subjects |
Toxicology ;
Phenobarbital ;
Liver neoplasms ;
Mice ;
Body weight ;
Deoxyribonucleic acids ;
Tables(Data) ;
Reprints ;
Ethoxyresorufin-O-deethylase ;
Carcinogenesis ;
Organ weight ;
Liver microsomes ;
Cytochrome P-450 ;
Amino N-demethylase
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB90-199415 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
11p |
Abstract |
A strain specificity has been demonstrated for the effect of subsequent administration of phenobarbital, in which diethylnitrosamine-initiated hepatocarcinogenesis was promoted in C3H mice, inhibited in B6C3F1 (C57BL X C3H) and not affected in C57BL mice. Therefore, the study examined in these three strains of mice and in C3B6F1 (C3H X C57BL) mice the effect of phenobarbital administered in their drinking water for 4 days or 28 days. The liver weight to body weight ratio was increased by phenobarbital in all types of mice. Microsomal protein concentrations were increased in C57BL mice after 28 days of treatment, in C3H after both 4 days and 28 days and in B6C3F1 after 4 days of treatment. No effect upon microsomal protein content was observed in C3B6F1 mice. DNA content was increased in C3H mice, both in 4 days and 28 days of treatment groups, while the other strains showed either a decrease or no difference from control. DNA synthesis was elevated in all strains of mice after 4 days of treatment with phenobarbital, however, after 28 days of treatment there was either a much reduced increase (C57B1 and C3B6F1) or no difference (C3H and B6C3F1) from controls. In all four types of mice after 4 and 28 days of treatment, phenobarbital increased the concentration of cytochrome P-450, the activity of aminopyrine-N-demethylase and 7-ethoxyresorufin-O-deethylase and the oxidation of testosterone. The oxidative metabolites of testosterone were similar in the four types of mice. |
Supplementary Notes |
Pub. in Cancer Letters, v48 p43-51 Oct 89. Prepared in cooperation with Medical Coll. of Ohio at Toledo, and Environmental Health Research and Testing, Inc., Cincinnati, OH. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Comparison of the Effects of Acute and Subacute Treatment of Phenobarbital in Different Strains of Mice. |
Category Codes |
57Y |
NTIS Prices |
PC A03/MF A01 |
Primary Description |
600/10 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
014420245 |
Origin |
NTIS |
Type |
CAT |