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OLS Field Name OLS Field Data
Main Title DNA (Deoxyribonucleic Acid) Adsorption to Soils and Sediments.
Author Ogram, A. ; Sayler, G. S. ; Gustin, D. ; Lewis, R. J. ;
CORP Author Tennessee Univ., Knoxville.;Environmental Research Lab., Gulf Breeze, FL.;National Institutes of Health, Bethesda, MD.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-89/038;
Stock Number PB90-103573
Additional Subjects Sediments ; Absorption ; Deoxyribonucleic acids ; Soil chemistry ; Ionic strength ; PH ; Extraction ; Montmorillonite ; Sands ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB90-103573 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 03/10/1990
Collation 5p
Abstract Deoxyribonucleic acid (DNA) adsorption of five soils, an acid-washed sand, and a lake sediment was investigated. All DNA at environmentally relevant concentrations was adsorbed by soils containing a significant amount of montmorillonite at low to neutral pH values. Studies on the effects of DNA molecular size on adsorption to sand and a sandy soil were described by the Freundlich isotherm model (r2 >0.85), and revealed that the higher the molecular weight, the more the adsorption. The effects of ionic strength (as sodium phosphate buffer) on adsorption showed that adsorption decreases as PO4 increases. Organic carbon was found to play a relatively minor role in the adsorption of DNA to these soils. A scheme for the extraction of DNA from soils was also developed. (Copyright 1988 American Chemical Society.)
Supplementary Notes Pub. in Environmental Science and Technology, v22 n8 p982-984 Aug 88. Sponsored by Environmental Research Lab., Gulf Breeze, FL., and National Institutes of Health, Bethesda, MD.
NTIS Title Notes Journal article.
Title Annotations Reprint: DNA (Deoxyribonucleic Acid) Adsorption to Soils and Sediments.
Category Codes 57F; 68C
NTIS Prices PC A02/MF A01
Primary Description 600/04
Document Type NT
Cataloging Source NTIS/MT
Control Number 932616992
Origin NTIS
Type CAT