Main Title |
Toxic Interactions between Carbon Tetrachloride and Chloroform in Cultured Rat Hepatocytes. |
Author |
Lamb, R. G. ;
Borzelleca, J. F. ;
Condie, L. W. ;
Gennings, C. ;
|
CORP Author |
Virginia Commonwealth Univ., Richmond. Dept. of Pharmacology and Toxicology.;Health Effects Research Lab., Cincinnati, OH. |
Publisher |
c1989 |
Year Published |
1989 |
Report Number |
EPA-R812558; EPA/600/J-89/342; |
Stock Number |
PB90-201054 |
Additional Subjects |
Toxicity ;
Carbon tetrachloride ;
Chloroform ;
Rats ;
Mitochondria ;
Graphs(Charts) ;
Tables(Data) ;
Reprints ;
Liver ;
Phosphatidycholines ;
Cultured cells ;
Cell membrane
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB90-201054 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
10p |
Abstract |
Primary cultures of adult rat hepatocytes were incubated (1.5-16 hr) with various concentrations of CCl4 (= or < 0.5 mM) and/or CHCl3 (= or < 2.5 mM). Agent-dependent alterations in hepatocyte functions were assessed by measuring (3H)choline incorporation into phosphatidylcholine (endoplasmic reticulum), MTT (tetrazolium salt) reduction (mitochondria), and AST release into medium (plasma membrane). Cultured hepatocytes incubated with 0.5 mM CCl4 displayed a significant (p = or < 0.001) and rapid (1.5 hr) reduction (40%) in endoplasmic reticulum function that preceded significant (p = or < 0.001) alterations in mitochondria (6-16 hr) and plasma membrane (6-16 hr) functions, CCl4-dependent alterations in liver cell functions are a result of CCl4 bioactivation since metyrapone inhibits the CCl4-mediated changes in cell functions. Response surface methods (RSM) were used to determine the influence of combinations of CCl4 and CHCl3 on liver cell MTT reduction and (3H)choline incorporation. Regression coefficients were determined for CCl4, CHCl3, and CCl4-CHCl3. All results were significant (p < 0.0001) and implied that CCl4 was a more potent hepatotoxin in vitro than CHCl3. The RSM analysis also suggested that combinations of CHCl3 and CCl4 have greater than additive effects on MTT reduction and (3H)choline incorporation. (Copyright (c) 1989 Academic Press, Inc.) |
Supplementary Notes |
Pub. in Fundamental and Applied Toxicology, v101 n1 p106-113 Oct 89. Sponsored by Health Effects Research Lab., Cincinnati, OH. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Toxic Interactions between Carbon Tetrachloride and Chloroform in Cultured Rat Hepatocytes. |
Category Codes |
57Y |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/10 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
016616798 |
Origin |
NTIS |
Type |
CAT |