||Effect of Pentachlorophenol on the Activation of 2,6-Dinitrotoluene to Genotoxic Urinary Metabolites in CD-1 Mice: A Comparison of GI Enzyme Activities and Urine Mutagenicity.
George, S. E. ;
Chadwick, R. W. ;
Creason, J. P. ;
Kohan, M. J. ;
Dekker, J. P. ;
||Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Metabolic activation ;
Salmonella typhimurium ;
Mutagenicity tests ;
Organ weight ;
Multivariate analysis ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||2,6-Dinitrotoluene (2,6-DNT) and pentachlorophenol (PCP) are used for industrial purposes and are found in the environment as hazardous contaminants. Because concurrent exposure to both compounds can occur, it is of interest to determine if organochlorine compounds potentiate the effect of nitroaromatic chemicals. CD-1 mice were treated with PCP (42.8 mg/kg) for 4 weeks. On weeks 1,2, and 4 after the initial PCP dose, mice were treated p.o. with 2,6-DNT (75 mg/kg) and 24 hr urines were collected. After concentration, the urines were tested for their mutagenic activity in Salmonella typhimurium strain TA98 without metabolic activation in a microsuspension bioassay. A significant increase (P < .05) in mutagenicity was observed in urines from mice treated with 2,6-DNT alone and in combination with PCP. By week 4, mice that received both 2,6-DNT and PCP excreted urine that was more mutagenic than that from animals which received only 2,6-DNT. At weeks 2 and 4, mice were sacrificed and intestinal enzyme activities (nitroreductase, azo reductase, Beta-glucuronidase, dechlorinase, and dehydrochlorinase) were quantitated. (Copyright (c) 1991 Wiley-Liss, Inc.)
||Pub. in Molecular and Environmental Mutagenesis, v18 n4 p92-101 Aug 91. See also PB89-216535. Prepared in cooperation with Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
|NTIS Title Notes
||Reprint: Effect of Pentachlorophenol on the Activation of 2,6-Dinitrotoluene to Genotoxic Urinary Metabolites in CD-1 Mice: A Comparison of GI Enzyme Activities and Urine Mutagenicity.
||PC A03/MF A01