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OLS Field Name OLS Field Data
Main Title S9-Dependent Activation of 1-Nitropyrene and 3-Nitrofluoranthene in Bacterial Mutagenicity Assays.
Author Ball, L. M. ; Williams, K. ; Kohan, M. J. ; Lewtas, J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering.
Year Published 1985
Report Number EPA/600/D-86/003;
Stock Number PB86-144631
Additional Subjects Toxicology ; Bacteria ; Bioassay ; Nitrogen organic compounds ; Fluoranthene/nitro ; Pyrene/nitro ; Mutagenesis ; Salmonella typhimurium
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB86-144631 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 12p
Abstract Nitro-substituted polycyclic aromatic hydrocarbons (N02PAH) such as 1-nitropyrene (NP) and 3-nitrofluoranthene (3-NFA), both widespread environmental mutagens, generally require activation by bacterial nitroreductases for maximal expression of their mutagenicity in the Ames Salmonella histidine reversion assay; addition of exogenous mammalian drug-metabolishing enzymes (S9 fraction) decreases their activity in the system. In contrast, in an assay of forward mutation (to 8-aza-guanine resistance) with Salmonella typhimurium strain TM677, without S9 NFA has relatively low activity (10 to 20 mutants per 10 to the 5th power survivors at 5 micrograms/ml) and NP only marginally doubles the background mutation rate. Addition of S9 protein enhances NP at 50 micrograms/ml and 80 to 100 x 10 to the minus 5 power for NFA at 5 to 10 micrograms/ml. Therefore generation of active genotoxic intermediates in the forward mutation system may proceed through metabolic pathways other than the previously-defined bacterial nitro-reduction. Previously-identified oxidative metabolites of NP are known to be mutagenic.
Supplementary Notes Prepared in cooperation with North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering.
PUB Date Free Form Dec 85
Category Codes 6T; 6M; 57Y; 57K
NTIS Prices PC A02/MF A01
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 607223398
Origin NTIS
Type CAT