||In vitro Culture of Postimplantation Hamster Embryos.
Ebron-McCoy, M. T. ;
Beyer, P. E. ;
Oglesby, L. A. ;
Kavlock, R. J. ;
||Health Effects Research Lab., Cincinnati, OH. ;Northrop Services, Inc., Research Triangle Park, NC.
Salicylic acids ;
In vitro analysis ;
Ovum implantation ;
Tissue culture ;
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||In vitro culture of intact rat and mouse embryos has been described extensively, but information on the culture of other species is sparse. The present study examined some culture requirements of early somite stage hamster embryos and assessed the embryotoxic effects of sodium salicylate (SS), a direct acting chemical and cyclophosphamide (CP), a proteratogen, on these embryos. Hamster embryos explanted on gestation days (GD) 8 and 9 were cultured in Waymouth's embryo-hepatocyte co-cultivation medium (WEHC), 70% McCoy's 5A medium-30% male rat serum (MMRS) or 100% male rat serum (MRS) for 24 hours under various oxygen concentrations. Embryos cultured GD 8 to 9 in the various media grew and differentiated much as they did in vivo, while embryos cultured GD 9 to 10 grew best in MMRS as compared to embryos at the same stage in vivo. Embryos exposed to SS in MMRS at concentrations of 250, 300, or 500 micrograms/ml showed dose related embryotoxicity which included CNS defects, absence of hind limb bud formation, and lack of axial rotation. Hamster embryos co-cultivated with pregnant hamster hepatocytes and treated with 2.5, 6.25 and 12.5 microgram/ml of CP, showed dose-dependent toxicity when compared to co-cultivated controls. Hamster embryos develop extensively in culture over a 24 hour period. This system may therefore provide a valuable tool for evaluating the species differences of a variety of potential teratogens and embryotoxins and allow the comparison of these embryotoxic effects between rat, mouse and hamster during similar stages of organogenesis. (Copyright (c) 1988 Pergamon Press.)
||Pub. in Reproductive Toxicology, v2 p31-36 Apr 88. Prepared in cooperation with Northrop Services, Inc., Research Triangle Park, NC.
|NTIS Title Notes
||Reprint: In vitro Culture of Postimplantation Hamster Embryos.
||PC A02/MF A01