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OLS Field Name OLS Field Data
Main Title Ca(2+)/Calmodulin-Dependent Protein Kinase Enriched in Cerebellar Granule Cells: Identification of a Novel Neuronal Calmodulin-Dependent Protein Kinase.
Author Ohmstede, C. A. ; Jensen, K. F. ; Sahyoun, N. E. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div. ;Burroughs Wellcome Co., Research Triangle Park, NC.
Publisher c1989
Year Published 1989
Report Number EPA/600/J-89/046;
Stock Number PB90-103623
Additional Subjects Calcium ; Rats ; Genetics ; Chemical analysis ; Isolation ; Reprints ; Protein kinases ; Calmodulin ; Brain chemistry ; Molecular cloning ; Immunohistochemistry ; Monoclonal antibodies ; Poly A ; Base sequence ; Immunoblotting
Holdings
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Status
NTIS  PB90-103623 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/10/1990
Collation 12p
Abstract The authors cloned a novel, brain-specific, Ca/calmodulin-dependent protein kinase from a rat lambda gt11 library which was probed for calmodulin-binding proteins. Antibodies raised against the b-galactosidase fusion protein recognized Mr 65000 and Mr 65000/67000 polypeptides from rat forebrain and cerebellum, respectively. Both polypeptides co-purified to homogeneity and phosphorylated synapsin I. The protein kinase is coded for by two poly A+RNAs at 2.0 kb and 3.5 kb which probably derive from a single gene. A 1.3 kbp cDNA insert contained 477 bp of protein coding sequence which included a calmodulin-binding domain, but no further sequence similarities to other protein kinases. Immunohistochemical localization with a monospecific antibody revealed that the enzyme was expressed in specific subpopulations and was exceptionally enriched in the granule cells of the cerebellum.
Supplementary Notes Pub. in Jnl. of Biological Chemistry, v264 n10 p5866-5875, 5 Apr 89. Prepared in cooperation with Burroughs Wellcome Co., Research Triangle Park, NC.
NTIS Title Notes Journal article.
Title Annotations Reprint: Ca(2+)/Calmodulin-Dependent Protein Kinase Enriched in Cerebellar Granule Cells: Identification of a Novel Neuronal Calmodulin-Dependent Protein Kinase.
Category Codes 57F; 57B; 68G
NTIS Prices PC A03/MF A01
Primary Description 600/10
Document Type NT
Cataloging Source NTIS/MT
Control Number 932617045
Origin NTIS
Type CAT