Main Title |
Detection of Norwalk Virus in Stools by Enzyme Immunoassay. |
Author |
Herrmann, J. E. ;
Nowak, N. A. ;
Blacklow, N. R. ;
|
CORP Author |
Massachusetts Univ. Medical School, Worcester.;Health Effects Research Lab., Research Triangle Park, NC. |
Year Published |
1985 |
Report Number |
EPA/600/J-85/279; |
Stock Number |
PB86-159985 |
Additional Subjects |
Viruses ;
Radioimmunoassay ;
Gastroenteritis ;
Detection ;
Reprints ;
Enzyme immunoassay ;
Norwalk virus
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB86-159985 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
9p |
Abstract |
The development of a solid-phase microtiter enzyme immunoassay (EIA) for detection of Norwalk virus antigen in stool samples is described. The EIA was compared with a previously developed radioimmunoassay (RIA) for detection of Norwalk virus antigen in stools obtained from 30 volunteers who received Norwalk virus. The EIA detected viral antigen in stools from 17 of the volunteers and the RIA detected viral antigen in 15. Seroconversion was a more sensitive indicator of infection in some patients. However, two samples from volunteers who were clinically ill but did not show seroconversion to Norwalk virus were positive for Norwalk virus antigen by both immunoassays. This indicates that antigen detection may be important for use in epidemiological studies. Neither of the immunoassays gave positive reactions for stools known to contain enteric adenovirus, rotavirus, or Hawaii virus, or in stools from patients with acute diarrhea of unknown cause. The stability of the EIA reagents and ease of use should provide a means for more extensive testing for Norwalk virus in outbreaks of gastroenteritis. (Copyright (c) 1985 Alan R. Liss, Inc.) |
Supplementary Notes |
Pub. in Jnl. of Medical Virology 17, p127-133 1985. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Detection of Norwalk Virus in Stools by Enzyme Immunoassay. |
PUB Date Free Form |
c1985 |
Category Codes |
6E; 6M; 57E; 57K |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
611415753 |
Origin |
NTIS |
Type |
CAT |