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OLS Field Name OLS Field Data
Main Title Antigen Detection with Monoclonal Antibodies for the Diagnosis of Adenovirus Gastroenteritis.
Author Herrmann, J. E. ; Perron-Henry, D. M. ; Blacklow, N. R. ;
CORP Author Massachusetts Univ. Medical School, Worcester.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1987
Report Number EPA/600/J-87/215;
Stock Number PB88-170675
Additional Subjects Antigens ; Diagnosis ; Detection ; Reprints ; Monoclonal antibodies ; Adenovirus gastroenteritis
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NTIS  PB88-170675 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 7p
Abstract The authors have developed a monoclonal antibody-based enzyme immunoassay (EIA) for direct detection of enteric adenoviruses in stool specimens from patients with gastroenteritis. Tests specific for each of the enteric adenoviruses, adenovirus type 40 (Ad40) and type 41 (Ad41) were designed. The sensitivity of the EIA was compared to isolation of virus in Graham 293 cells from stools which contained particles of adenovirus morphology by electron microscopy. The standard for specificity was analysis of adenoviral genome profiles after digestion with SmaI endonuclease. The sensitivity was 95.8% (23/24) for Ad40 and 97.1% (34/35) for Ad41. The specificity was 95.7 (45/47) and 97.2% (35/36) respectively. The two type-specific monoclonal antibodies could be mixed in an EIA to identify enteric adenoviruses in stools without loss of reactivity in either type. The tests provide an effective and convenient means for rapid diagnosis and type-specific identification of enteric adenoviruses in gastroenteritis, and will substantially facilitate epidemiological studies of their infection.
Supplementary Notes Pub. in Jnl. of Infectious Diseases, v155 n6 p1167-1171 Jun 87. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
NTIS Title Notes Journal article.
Title Annotations Reprint: Antigen Detection with Monoclonal Antibodies for the Diagnosis of Adenovirus Gastroenteritis.
PUB Date Free Form c1987
Category Codes 57E; 57J
NTIS Prices PC A02/MF A01
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 812722522
Origin NTIS
Type CAT