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OLS Field Name OLS Field Data
Main Title Persistence of Lymphocytes with Dicentric Chromosomes Following Whole-Body X Irradiation of Mice.
Author Kilgerman, A. D. ; Halperin, E. C. ; Erexson, G. L. ; Honore, G. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Duke Univ. Medical Center, Durham, NC. Dept. of Radiology. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/431;
Stock Number PB91-171868
Additional Subjects Chromosome aberrations ; X-rays ; Lymphocytes ; Mice ; Kinetics ; Statistical analysis ; Metaphase ; Leukocytes ; Whole body irradiation ; Reprints ;
Holdings
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NTIS  PB91-171868 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 09/04/1991
Collation 8p
Abstract Thirty-six male C57B1/6 mice were whole-body x-irradiated with 3 Gy to generate lymphocytes with dicentric chromosomes to study the persistence of these lymphocytes in the spleen and peripheral blood to estimate the lifespan of mature B- and T-cells. Blood and spleen were removed from groups of four mice immediately after radiation exposure and on days 1, 3, 7, 14, 28, 56, and 12, thereafter. The initial frequencies of dicentric chromosomes with accompanying fragments observed in splenic T-cells (0.44), splenic B-cells (0.43), and peripheral blood lymphocyte cultures (0.48) initiated on day 0 were not significantly different. For both splenic and peripheral blood-T-lymphocytes, the frequency of cells containing dicentric chromosomes declined in an exponential manner following irradiation, with a 50% reduction in frequency occurring 14 days after exposure. In contrast, the frequency of B-cells containing dicentric chromosomes remained stable through day 7 but then declined precipitously between day 7 and 14 and remained relatively stable, although slightly above baseline, through day 112 post-exposure.
Supplementary Notes Pub. in Radiation Research, v124 p22-27 1990. Prepared in cooperation with Duke Univ. Medical Center, Durham, NC. Dept. of Radiology, and Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
NTIS Title Notes Journal article.
Title Annotations Reprint: Persistence of Lymphocytes with Dicentric Chromosomes Following Whole-Body X Irradiation of Mice.
Category Codes 57V; 57F
NTIS Prices PC A02/MF A01
Primary Description 600/10
Document Type NT
Cataloging Source NTIS/MT
Control Number 114324909
Origin NTIS
Type CAT