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OLS Field Name OLS Field Data
Main Title Estimating the Rate of Plasmid Transfer: An End-Point Method.
Author Simonsen, L. ; Gordon, D. M. ; Stewart, F. M. ; Levin., B. R. ;
CORP Author Massachusetts Univ., Amherst. Dept. of Zoology. ;Brown Univ., Providence, RI.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/551;
Stock Number PB92-129626
Additional Subjects Plasmids ; Escherichia coli ; Genetic conjugation ; Kinetics ; Computerized simulation ; Growth ; Reprints ;
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NTIS  PB92-129626 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 08/28/1992
Collation 9p
Abstract The document describes a method for determining the rate parameter of conjugative plasmid transfer that is based on single estimates of donor, recipient and transconjugant densities and the growth rate in exponential phase of the mating culture. The formula for estimating the plasmid transfer rate, gamma, was derived from a mathematical model describing cell growth and plasmid transfer in batch culture. Computer simulations were used to explore the sensitivity of the method to the realities of bacterial life, such as growth rate differences, plasmid segregation and transitory derepression of pilus synthesis. As predicted by the theory, mating experiments with the plasmid R1 in Escherichia coli K12 demonstrated that the estimate gamma is unaffected by cell density, donor: recipient ratio and mating time. Unlike previous techniques, the method allows investigations into the effect of environmental factors on plasmid transfer rates when these factors also influence population growth rates. To illustrate this, the authors examined the effect of temperature on the rate of plasmid transfer. (Copyright (c) 1990 SGM.)
Supplementary Notes Pub. in Jnl. of General Microbiology, v136 p2319-2325 1990. See also PB91-163816. Prepared in cooperation with Brown Univ., Providence, RI. Sponsored by Environmental Research Lab., Gulf Breeze, FL.
NTIS Title Notes Journal article.
Title Annotations Reprint: Estimating the Rate of Plasmid Transfer: An End-Point Method.
Category Codes 57F
NTIS Prices PC A02/MF A01
Primary Description 600/04
Document Type NT
Cataloging Source NTIS/MT
Control Number 207232341
Origin NTIS
Type CAT