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OLS Field Name OLS Field Data
Main Title Identification of fecal indicator bacteria isolates from an ice-covered river /
Author Sparrow, Elena B. ; Davenport, Charlotte V. ; Gordon, Ronald C.
Other Authors
Author Title of a Work
Davenport, Charlotte V.,
Gordon, Ronald C.,
CORP Author National Heart, Lung, and Blood Inst., Bethesda, MD.
Publisher Environmental Protection Agency, Office of Research and Development [Office of Air, Land, and Water Use], Corvallis Environmental Research Laboratory ; For sale by the National Technical Information Service,
Year Published 1979
Report Number EPA-600/3-79-073
Stock Number PB81-233017
OCLC Number 05470858
ISBN pbk.
Subjects Water--Microbiology. ; Ice--Microbiology. ; Water--Pollution. ; Water--Bacteriology ; Ice--Bacteriology
Additional Subjects Indicator species ; Bacteria ; Streams ; Coliform bacteria ; Enterobacteriaceae ; Escherichia coli ; Klebsiella ; Streptococcus ; Feces ; Bioindicators ; Water pollution detection
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EHAM  QR105.5.S63 Region 1 Library/Boston,MA 04/29/2016
EJAD  EPA 600/3-79-073 Region 3 Library/Philadelphia, PA 04/30/1993
EJBD ARCHIVE EPA 600-3-79-073 Headquarters Library/Washington,DC 04/10/2014
EJBD  EPA 600-3-79-073 Headquarters Library/Washington,DC 03/22/2016
EKAM  EPA 600/3-79-073 Region 4 Library/Atlanta,GA 05/13/1994
ELBD  EPA 600-3-79-073 AWBERC Library/Cincinnati,OH 03/06/2018
ESAD  EPA 600-3-79-073 Region 10 Library/Seattle,WA 03/23/2010
ESBD  EPA-6003-79-073 NHEERL/WED Library/Corvallis,OR 09/05/2017
NTIS  PB81-233017 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 01/01/1988
Collation vi, 13 ; 28 cm.
Abstract The membrane filter technique was used to enumerate the total coliform (TC), fecal coliform (FC), and fecal streptococcus (FS) populations at seven sample stations on an ice-covered river downstream from a major source of domestic pollution. From each membrane filter population (m-TC, m-FC, and m-FS), 210 typical colonies (30 per station) were selected for verification and biochemical differentiation of the component genera. The 210 m-TC isolates were Klebsiella pneumoniae (46.2%), Escherichia coli (20.5%), Enterobacter sp. (18.6%), other total coliforms (5.2%), and 9.5% which did not verify as total coliforms. Among these m-TC cultures, 114 were verified as fecal coliforms (gas production in EC broth at 44.5C). These 114 fecal coliforms were principally K. pneumoniae (53.5%) and E. coli (35.1%). In contrast, the 210 m-FC cultures were predominantly E. coli (77.6%), with K. pneumoniae (10.0%), other fecal coliforms (3.8%), and those not verified as fecal coliforms (8.6%). Of the 210 m-FS isolates, 167 were identified as enterococci, with 165 being Streptococcus faecalis biotypes. The results suggested the majority of these indicator bacteria originated from warm-blooded animal feces. Also, within each population, no overall differences in low temperature survival of the component genera were noted. However, the evidence does suggest that generic selectivity of the m-TC and m-FC techniques biases determination of the FC population composition, and that the m-FC technique underestimates the FC population density.
Notes July 1979. Includes bibliographical references.
Place Published Corvallis, Or. Springfield, Va.
Access Notes Also issued online.
Corporate Au Added Ent Corvallis Environmental Research Laboratory. Arctic Environmental Research Station.
Corp Au Ser Add Ent United States. Environmental Protection Agency. Ecological research series ; EPA-600/3-79-073.
PUB Date Free Form 1979
Series Title Untraced Ecological research series ; EPA-600/3-79-073
NTIS Prices PC A02/MF A01
BIB Level m
Medium unmediated
Content text
Carrier volume
Cataloging Source OCLC/T
OCLC Time Stamp 20180226121246
Language eng
SUDOCS Number EP 1.23:600/3-79-073
Origin OCLC
OCLC Rec Leader 02921cam 2200601Ii 45010