||Initial-Phase Optimization for Bioremediation of Munition Compound-Contaminated Soils.
Funk, S. B. ;
Roberts, D. J. ;
Crawford, D. L. ;
Crawford, R. L. ;
||Idaho Univ., Moscow. Dept. of Bacteriology and Biochemistry.;Environmental Research Lab., Athens, GA. Office of Research and Development.
Soil pollution ;
Remedial action ;
Anaerobic processes ;
Nitrogen organic compounds ;
Nitro compounds ;
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||We examined the bioremediation of soils contaminated with the munition compounds 2,4,6-trinitrotoluene (TNT), hexahydro-1,3,5-trinitro-1,3,5-triazine, and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetraacocine by a procedure that produced anaerobic conditions in the soils and promoted biodegradation of nitroaromatic contaminants. This procedure consisted of flooding the soils with 50 mM phosphate buffer, adding starch as a supplemental carbon substrate, and incubating under static conditions. Aerobic heterotrophs, present naturally in the soil or added as an inoculum, quickly removed the oxygen from the static cultures, creating anaerobic conditions. Removal of parent TNT molecules from the soil cultures by the strictly anaerobic microflora occurred within 4 days.
||Pub. in Applied and Environmental Microbiology, v59 n7 p2171-2177 Jul 93. Sponsored by Environmental Research Lab., Athens, GA. Office of Research and Development.
|NTIS Title Notes
||Reprint: Initial-Phase Optimization for Bioremediation of Munition Compound-Contaminated Soils.
||68; 79A; 48E
||PC A02/MF A01