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RECORD NUMBER: 23 OF 47

OLS Field Name OLS Field Data
Main Title Glutathione and Mercapturic Acid Conjugations in the Metabolism of Naphthalene and 1-Naphthyl N-Methylcarbamate (Carbaryl).
Author Chen, Ke-Chang ; Dorough, H. Wyman ;
CORP Author Kentucky Univ., Lexington.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1979
Report Number EPA-R-805143; EPA-600/J-79-120;
Stock Number PB81-192049
Additional Subjects Glutathione ; Naphthalene ; Rats ; Liver ; Metabolism ; In vitro analysis ; In vivo analysis ; Carbamates ; Nitrogen organic compounds ; Reprints ; Carbaryl ; Cysteine/N-acetyl ; Carbamic acid/naphthyl-N-methyl
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NTIS  PB81-192049 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation 23p
Abstract The formation of glutathione (GSH) conjugate in the detoxification of (1-14C)-naphthalene and (naphthyl-14C)-carbaryl was investigated using rat liver homogenate. The mercapturic acid conjugate in rats was also investigated by collection of urine after intraperitoneal injection of 14C substrates. The formation of water-soluble metabolites in vitro from naphthalene was dependent on the amount of glutathione added, but this was not seen in carbaryl metabolism. In vitro, the metabolism of (1-14C)-naphthalene produced 50% GSH conjugate in the incubation mixture, whereas in vivo the metabolism of this compound produced 65% mercapturic acid conjugate in the urine. There was no evidence of GSH or mercapturic acid conjugate in the metabolism of (naphthyl-14C)-carbaryl in vitro and in vivo. This conclusion was made by comparing the nature and chemical characteristics of GSH and mercapturic acid conjugates formed in (1-14C)-naphthalene metabolism. With the aid of the specific enzyme (e.g. B-glucuronidase and sulfatase) and acid hydrolysis, the water-soluble metabolites of (naphthyl-14C)-carbaryl were tentatively recognized as glucuronide or sulfate conjugated mainly with 5,6-dihydro-5,6-dihydroxycarbaryl or N-hydroxymethyl carbaryl and their hydrolytic products. This data demonstrated that the substituent group on the naphthalene molecule may affect the significance of GSH conjugation.
Availability Notes Pub. in Drug and Chemical Toxicology, v2 n4 p331-354 1979.
NTIS Title Notes Journal article.
Title Annotations Reprint: Glutathione and Mercapturic Acid Conjugations in the Metabolism of Nahthalene and 1-Naphthyl N-Methylcarbamate (Carbaryl).
PUB Date Free Form c1979
Category Codes 6B; 57A
NTIS Prices Not available NTIS
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 323797227
Origin NTIS
Type CAT