Main Title |
Mutants of 'Pseudomonas cepacia' G4 Defective in Catabolism of Aromatic Compounds and Trichloroethylene. |
Author |
Shields, M. S. ;
Montgomery, S. O. ;
Cuskey, S. M. ;
Chapman., P. J. ;
|
CORP Author |
Environmental Research Lab., Gulf Breeze, FL. |
Publisher |
c1991 |
Year Published |
1991 |
Report Number |
EPA/600/J-91/337 ;CONTRIB-730; |
Stock Number |
PB92-129741 |
Additional Subjects |
Mutations ;
Toluene ;
Trichloroethylene ;
Aromatic compounds ;
Metabolism ;
Catalysis ;
Mutagenesis ;
Gas chromatography ;
Phenotype ;
Hydroxylation ;
Oxidation ;
Biodeterioration ;
Reprints ;
Pseudomonas cepacia
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB92-129741 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
9p |
Abstract |
Pseudomonas cepacia G4 possesses a novel pathway of toluene catabolism that is shown to be responsible for the degradation of trichloroethylene (TCE). The pathway involves conversion of toluene via o-cresol to 3-methylcatechol. In order to determine the enzyme of toluene degradation that is responsible for TCE degradation, chemically induced mutants, blocked in the toluene ortho-monooxygenase (TOM) pathway of G4, were examined. Mutants of the phenotypic class designated TOM A(-) were all defective in their ability to oxidize toluene, o-cresol, m-cresol, and phenol, suggesting that a single enzyme is responsible for conversion of these compounds to their hydroxylated products (3-methylcatechol from toluene, o-cresol, and m-cresol and catechol from phenol) in the wild type. Mutants of this class did not degrade TCE. Two other mutant classes which were blocked in toluene catabolism, TOM B(-), which lacked catechol-2,3-dioxygenase, and TOM C(-) which lacked 2-hydroxy-6-oxoheptadienoic acid hydrolase activity, were fully capable of TCE degradation. Therefore, TCE degradation is directly associated with the monooxygenation capability responsible for toluene, cresol, and phenol hydroxylation. (Copyright (c) 1991, American Society for Microbiology.) |
Supplementary Notes |
Pub. in Applied and Environmental Microbiology, v57 n7 p1935-1941 Jul 91. See also PB90-129537. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Mutants of 'Pseudomonas cepacia' G4 Defective in Catabolism of Aromatic Compounds and Trichloroethylene. |
Category Codes |
57K; 57B; 57F |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/04 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
205223989 |
Origin |
NTIS |
Type |
CAT |