||Copper Toxicity to Sperm, Embryos and Larvae of Topsmelt 'Atherinops affinis', with Notes on Induced Spawning.
Anderson, B. S. ;
Middaugh, D. P. ;
Hunt, J. W. ;
Turpen., S. L. ;
||Environmental Research Lab., Gulf Breeze, FL. ;California Univ., Santa Cruz. ;California State Dept. of Fish and Game, Monterey. Marine Pollution Studies Lab.
Water pollution effects(Animals) ;
Marine fishes ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||Topsmelt, Atherinops affinis, were induced to spawn repeatedly in the laboratory using a combination of environmental cues. Temperature spikes appeared to be the most important factor to induce spawning. Egg production peaked four days after a 2C increase in water temperature, and declined thereafter. A series of static toxicity tests compared the relative sensitivity of topsmelt sperm, embryos, and larvae to copper chloride. Of the three developmental stages compared, sperm were more sensitive than embryos, and embryos were more sensitive than larvae. The mean EC50 from four separate 48-h fertilization experiments was 109 micrograms copper/liter. The mean EC50 from three, 12-day embryo development development tests was 142-147 micrograms copper/liter, depending on the endpoint used. The mean LC50 from three, 96-h larval mortality tests was 238 micrograms copper/liter. Topsmelt are amenable to laboratory culture and are a promising eastern Pacific toxicity test species. (Copyright (c) 1991 Elsevier Science Publishers Ltd, England.)
||Pub. in Marine Environmental Research, v31 p17-35 1991. See also PB85-227023. Prepared in cooperation with California Univ., Santa Cruz, and California State Dept. of Fish and Game, Monterey. Marine Pollution Studies Lab.
|NTIS Title Notes
||Reprint: Copper Toxicity to Sperm, Embryos and Larvae of Topsmelt 'Atherinops affinis', with Notes on Induced Spawning.
||68D; 57Y; 47D
||PC A03/MF A01