||Catalase and Superoxide Dismutase of Root-Colonizing Saprophytic Fluorescent Pseudomonads.
Katsuwon, J. ;
Anderson, A. J. ;
||Utah Agricultural Experiment Station, Logan.;Environmental Research Lab., Gulf Breeze, FL.
Superoxide dismutase ;
Pseudomonas putida ;
Pseudomonas fluorescens ;
Bean plants ;
Microbial colony count ;
Polyacrylamide gel electrophoresis ;
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||Increased specific activities of catalase but not superoxide dismutase were observed during growth of these bacteria on components washed from root surfaces. The specific activities of both enzymes were also regulated during contact of these bacteria with intact bean roots. Increased superoxide dismutase and decreased catalase activities were observed rapidly, by 10 min upon inoculation of cells onto intact bean roots. Catalase specific activity increased with time to peak at 12 h before declining. By 48 h, the cells displayed this low catalase but maintained high superoxide dismutase specific activities. Catalase with a low specific activity and a high superoxide dismutase activity also were present in extracts of cells obtained from 7-day-old roots colonized from inoculum applied to seed. The specific activity of superoxide dismutase of root-contacted cells was about fourfold-higher in comparison to cells grown on rich medium, whereas the specific activity for catalase was reduced about fivefold. A single catalase isozyme, isozyme A, and one isozyme of superoxide dismutase, isozyme 1, were detected during growth of the bacteria on root surface components and during exposure of cells to intact bean roots for 1 h. An additional catalase, isozyme B, was detected from bacteria after exposure to the intact bean roots for 12 h. Catalase isozyme A and superoxide dismutase isozyme 1 were located in the cytoplasm and catalase band B was located in the membrane of P. putida. (Copyright (c) 1990, American Society for Microbiology.)
||Pub. in Applied and Environmental Microbiology, v56 n11 p3576-3582 Nov 90. See also PB90-264847. Sponsored by Environmental Research Lab., Gulf Breeze, FL.
|NTIS Title Notes
||Reprint: Catalase and Superoxide Dismutase of Root-Colonizing Saprophytic Fluorescent Pseudomonads.
||PC A02/MF A01