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OLS Field Name OLS Field Data
Main Title Efficacy of beta-Glucuronidase Assay for Identification of 'Escherichia coli' by the Defined-Substrate Technology.
Author Rice, E. W. ; Allen, M. J. ; Edberg, S. C. ;
CORP Author Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. ;American Water Works Association Research Foundation, Denver, CO. ;Yale Univ., New Haven, CT. Dept. of Lab. Medicine.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/149;
Stock Number PB91-109876
Additional Subjects Escherichia coli ; Bioassay ; Enzymes ; Water pollution ; Feces ; Humans ; Livestock ; Reprints ; Glucuronidase ; Drinking water
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB91-109876 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/04/1991
Collation 5p
Abstract In 1976, Kilian and Bulow described the association of B-Glucuronidase with the genus Escherichia (97% positive) and suggested that a B-Glucuronidase assay would be a useful identification test. Since that report, papers about the sensitivity and specificity of this enzyme for the identification of Escherichia coli from clinical sources, food, seawater, potable-water supplies, and various environmental sources have appeared. A study was undertaken to determine the efficacy and specificity of the defined-substrate technology B-glucuronidase (Colilert) assay for the identification of this species from fecal samples. A total of 460 human, 105 cow, and 55 horse E. coli isolates were tested. Results showed 95.5% B-Glucuronidase-positive isolates in 24 h and 99.5% positive after 28 h of incubation. Only one E. coli isolate was negative. There were no significant differences in the percentage of B-Glucuronidase-positive isolates among the human or animal isolates. There were no non-E. coli isolates that were positive. All subjects carried B-Glucuronidase-positive E. coli. (Copyright (c) 1990 American Society for Microbiology.)
Supplementary Notes Pub. in Applied and Environmental Microbiology, v56 n5 p1203-1205 May 90. Prepared in cooperation with American Water Works Association Research Foundation, Denver, CO., and Yale Univ., New Haven, CT. Dept. of Lab. Medicine.
NTIS Title Notes Journal article.
Title Annotations Reprint: Efficacy of beta-Glucuronidase Assay for Identification of 'Escherichia coli' by the Defined-Substrate Technology.
Category Codes 68D; 57K
NTIS Prices PC A01/MF A01
Primary Description 600/14
Document Type NT
Cataloging Source NTIS/MT
Control Number 034834779
Origin NTIS
Type CAT