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OLS Field Name OLS Field Data
Main Title Growth Characteristics, Morphology, and Phospholipid Composition of Human Type 2 Pulmonary Alveolar Cells Grown in a Collagen-Free Microenvironment.
Author Milo, G. E. ; Ackerman, G. A. ; Sanders, R. L. ;
CORP Author Ohio State Univ., Columbus. ;Tufts Univ., Boston, MA. School of Medicine.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1984
Report Number EPA-R-806638; EPA/600/J-84/378;
Stock Number PB86-211935
Additional Subjects Growth ; Morphology ; Phospholipids ; Pulmonary alveolar cells
Library Call Number Additional Info Location Last
NTIS  PB86-211935 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 16p
Abstract Human lung epithelial cells have been cultured and characterized for phospholipid content. Any residual fibroblasts were removed by selective trypsinization within the first 48 hours in culture. Epithelial cells were serially subpassaged when cultures reached ca. 80% confluency. Electron microscopic evaluation of both monolayers and cell suspensions showed cultures to be composed of a single cell type. These cells had a microvillar free surface and were attached by descosomes which characterized them as epithelial. They contained lamellar inclusions which is typical of type II cells. Fetal lung (age 18-20 weeks) cell suspension processed for electron microscopy prior to culturing showed cells to be undifferentiated, epithelial-like with small microvilli along cell borders and desmosomes at cell cultured epithelial cell deconstrated that the lamellar inclusions were not observed in these cells. Ultrastructural studies of the cultured epithelial cell demonstrated that the lamellar inclusions had a slightly positive reaction when tested for acid phosphatase.
Supplementary Notes Pub in In Vitro, v20 n12 p899-911 Dec 84. Prepared in cooperation with Tufts Univ., Boston, MA. School of Medicine. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
NTIS Title Notes Journal article.
PUB Date Free Form c1984
Category Codes 6C; 57F
NTIS Prices PC A02/MF A01
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 624111209
Origin NTIS
Type CAT