| Main Title |
Use of Immunofluorescence and Phase-Contrast Microscopy for Detection and Identification of 'Giardia' Cysts in Water Samples. |
| Author |
Sauch, J. F. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. |
| Year Published |
1985 |
| Report Number |
EPA/600/J-85/365; |
| Stock Number |
PB86-177771 |
| Additional Subjects |
Cysts ;
Disease vectors ;
Surface waters ;
Water pollution ;
Monitoring ;
Reprints ;
Giardiasis ;
Fluorescent antibody technique ;
Waterborne diseases
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB86-177771 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
6p |
| Abstract |
A method was developed in which indirect immunofluorescence and phase-contrast microscopy are used for rapid detection and identification of Giardia cysts in raw and finished water supplies. When anti-Giardia cyst antiserum and fluorescein conjugate were applied to known Giardia cysts on membrane filters, the cysts fluoresced bright green when they were illuminated by UV light. This procedure permitted individual cysts to be quickly located even in samples heavily contaminated with other microorganisms and debris. The identity of presumptive Giardia cysts located in this way could then be confirmed by observing characteristic internal morphological features with phase-contrast microscopy. With this method, Giardia cysts were detected and their identities were confirmed in samples taken from raw and finished surface water supplies during several recent outbreaks. |
| Supplementary Notes |
Pub. in Applied and Environmental Microbiology, v50 n6 p1434-1438 Dec 85. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Use of Immunofluorescence and Phase-Contrast Microscopy for Detection and Identification of 'Giardia' Cysts in Water Samples. |
| PUB Date Free Form |
1985 |
| Category Codes |
6E; 44G; 68D; 57N |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
614223802 |
| Origin |
NTIS |
| Type |
CAT |
