| Main Title |
Natural Transformation of a Marine 'Vibrio' Species by Plasmid DNA. |
| Author |
Jeffrey, W. H. ;
Paul, J. H. ;
Stewart, G. J. ;
|
| CORP Author |
Environmental Research Lab., Gulf Breeze, FL. ;University of South Florida, St. Petersburg. |
| Publisher |
c1990 |
| Year Published |
1990 |
| Report Number |
EPA/600/J-90/377; |
| Stock Number |
PB91-163907 |
| Additional Subjects |
Vibrio ;
Water microbiology ;
Marine biology ;
Deoxyribonucleic acids ;
Plasmids ;
Chromosomes ;
Southern blotting ;
Autoradiography ;
Reprints ;
Genetic transformation
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB91-163907 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
12p |
| Abstract |
A series of thirty marine and estuarine bacterial isolates was examined for the ability to naturally transform with plasmid DNA. One isolate from Tampa Bay, Florida, identified as Vibrio parahaemolyticus, successfully incorporated and maintained the broad host range plasmid pKT230 in both filter transformation assays and sterile sediment microcosms, at frequencies ranging from 0.3 to 3.1 x 10 to the minus eighth power transformants per recipient. Transformation occurred without deletions and transformation of multimeric forms of the plasmid. Results suggest that natural transformation may be one mechanism by which estuarine bacteria acquire antibiotic resistance plasmids. |
| Supplementary Notes |
Pub. in Microbial Ecology, v19 p259-268 1990. Prepared in cooperation with University of South Florida, St. Petersburg. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Natural Transformation of a Marine 'Vibrio' Species by Plasmid DNA. |
| Category Codes |
57K; 57F; 47D |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/04 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
113027723 |
| Origin |
NTIS |
| Type |
CAT |
