| Main Title |
Selection of a 'Pseudomonas cepacia' Strain Constitutive for the Degradation of Trichloroethylene. |
| Author |
Shields, M. S. ;
Reagin, M. J. ;
|
| CORP Author |
Technical Resources, Inc., Gulf Breeze, FL. ;University of West Florida, Pensacola. Center for Environmental Diagnostics and Bioremediation.;Environmental Research Lab., Gulf Breeze, FL. |
| Publisher |
c1992 |
| Year Published |
1992 |
| Report Number |
EPA-68-03-3479; EPA/600/J-93/068 ; CONTRIB-790 |
| Stock Number |
PB93-169027 |
| Additional Subjects |
Biodeterioration ;
Trichloroethylene ;
Growth ;
Toluene ;
Phenols ;
DNA insertion elements ;
Mutation ;
Chlorine organic compounds ;
Reprints ;
Pseudomonas cepacia
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB93-169027 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
9p |
| Abstract |
Tn5 insertion mutants of Pseudomonas cepacia G4 that were unable to degrade trichloroethylene (TCE), toluene, or phenol or to transform m-trifluoromethyl phenol (TFMP) to 7,7,7-trifluoro-2-hydroxy-6-oxo-2,4-heptadienoic acid (TFHA) were produced. Spontaneous reversion to growth on phenol or toluene as the sole source of carbon was observed in one mutant strain, G4 5223, at a frequency of about .0004 per generation. One such revertant, G4 5223-PR1, metabolized TFMP to TFHA and degraded TCE. Unlike wild-type G4, G4 5223-PR1 constitutively metabolized both TFMP and TCE without aromatic induction. G4 5223-PR1 also degraded cis-1,2-dichloroethylene, trans-1,2-dichloroethylene, and 1,1-dichloroethylene and oxidized naphthalene to alpha naphthol constitutively. G4 5223-PR1 exhibited a slight retardation in growth rate at TCE concentrations of greater than or equal to 530 microM, whereas G4 (which was unable to metabolize TCE under the same noninducing growth conditions) remained unaffected. The constitutive degradative phenotype of G4 5223-PR1 was completely stable through 100 generations of nonselective growth. (Copyright (c) 1992, American Society for Microbiology.) |
| Supplementary Notes |
See also PB90-129537. Prepared in cooperation with University of West Florida, Pensacola. Center for Environmental Diagnostics and Bioremediation. Sponsored by Environmental Research Lab., Gulf Breeze, FL. |
| Availability Notes |
Pub. in Applied and Environmental Microbiology, v58 n12 p3977-3983 Dec 92. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Selection of a 'Pseudomonas cepacia' Strain Constitutive for the Degradation of Trichloroethylene. |
| Category Codes |
57K; 57B; 57F |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/04 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
314124373 |
| Origin |
NTIS |
| Type |
CAT |
