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Main Title Cloning and Expression of the catA and catBC Gene Clusters from 'Pseudomonas aeruginosa' PAO.
Author Kukor, J. J. ; Olsen, R. H. ; Ballou, D. P. ;
CORP Author Michigan Univ., Ann Arbor. Medical School.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1988
Year Published 1988
Report Number EPA-R-812679; EPA/600/J-88/529;
Stock Number PB90-264920
Additional Subjects Mutations ; Catalysis ; Reprints ; Pseudomonas aeruginosa ; Reiterated genes ; Molecular cloning ; Gene expression ; Plasmids ; Restriction mapping ; Transfection ; Recombination(Genetics) ; Bacterial DNA
Library Call Number Additional Info Location Last
NTIS  PB90-264920 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
Abstract A 9.9-kilobase (kb) BAMIII restriction endonuclease fragment encoding the catA and catBC gene clusters was selected from a gene bank of the Pseudomonas aeruginosa PAO1c chromosome. The catA, catB, and catC genes encode enzymes that catalyze consecutive reactions in the catechol branch of the beta-ketoadipate pathway. A recombinant plasmid, pRo1783, which contains the 9.9-kb BAMIII restriction fragment complemented P. aeruginosa, mutants with lesions in the catA, catB, or catC gene; however, this fragment of chromosomal DNA did not contain any other catabolic gene which had been placed near the catA or catBC cluster based on cotransducibility of the loci. Restriction mapping, deletion subcloning, and complementation analysis showed that the order of the genes on the cloned chromosomal DNA fragment is catA, catB, catC. (Copyright (c) 1988, American Society for Microbiology.)
Supplementary Notes Pub. in Jnl. of Bacteriology, v170 n18 p4458-4465 Oct 88. Sponsored by Environmental Research Lab., Gulf Breeze, FL.
NTIS Title Notes Journal article.
Title Annotations Reprint: Cloning and Expression of the catA and catBC Gene Clusters from 'Pseudomonas aeruginosa' PAO.
Category Codes 57K; 57F
NTIS Prices PC A02/MF A01
Primary Description 600/04
Document Type NT
Cataloging Source NTIS/MT
Control Number 028533169
Origin NTIS
Type CAT