| Main Title |
Enzyme-Linked Immunosorbent Assays for Detection and Quantitation of the Entomocidal Parasporal Crystalline Protein of 'Bacillus thuringiensis' subspp. 'kurstaki' and 'israelensis'. |
| Author |
Wie, S. I. ;
Andrews, R. E. ;
Hammock, B. D. ;
Faust, R. M. ;
Bulla, L. A. ;
|
| CORP Author |
California Univ., Davis. ;Kansas State Univ., Manhattan. ;Department of Agriculture, Beltsville, MD.;Health Effects Research Lab., Research Triangle Park, NC. |
| Year Published |
1982 |
| Report Number |
EPA-R-806447; EPA/600/J-82/469; |
| Stock Number |
PB87-115358 |
| Additional Subjects |
Toxins ;
Bacteria ;
Reprints ;
Immunosorbent assays ;
Bacillus thuringiensis
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB87-115358 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
6p |
| Abstract |
An enzyme-linked immunosorbent assay (ELISA) was used to detect and quantitate the parasporal crystal toxins of Bacillus thuringiensis subspp. kurstaki and israelensis. The ELISA method described is extremely sensitive, accurate, and highly specific. Using the technique, crystalline insecticidal proteins from several subspecies of B. thuringiensis were compared. The dipteran crystal toxin produced by B. thuringiensis subsp. israelensis was shown to share few epitopes with the lepidopteran toxin from subspecies kurstaki, tolworthi, berliner and alesti. |
| Supplementary Notes |
Pub. in Applied and Environmental Microbiology, v43 n4 p891-894 Apr 82. Prepared in cooperation with Kansas State Univ., Manhattan, and Department of Agriculture, Beltsville, MD. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Enzyme-Linked Immunosorbent Assays for Detection and Quantitation of the Entomocidal Parasporal Crystalline Protein of 'Bacillus thuringiensis' subspp. 'kurstaki' and 'israelensis'. |
| PUB Date Free Form |
c1982 |
| Category Codes |
57B |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
702814043 |
| Origin |
NTIS |
| Type |
CAT |
