| Main Title |
Non-Random Cell Killing in Cryopreservation: Implications for Performance of the Battery of Leukocyte Tests (BLT), 1. Toxic and Immunotoxic Effects. |
| Author |
Strauss., G. H. S. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. |
| Publisher |
c1991 |
| Year Published |
1991 |
| Report Number |
EPA/600/J-90/508; |
| Stock Number |
PB91-199943 |
| Additional Subjects |
Cell survival ;
Cryopreservation ;
Longitudinal studies ;
T lymphocytes ;
Cell line ;
Mutagens ;
Toxins ;
In vivo analysis ;
In vitro analysis ;
Reprints ;
Battery of leukocyte tests
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB91-199943 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
17p |
| Abstract |
To eliminate between-tests error in longitudinal studies, for specimen sharing, convenient scheduling, etc., it is necessary to freeze freshly separated leukocytes as well as non-transformed, continuous T lymphocyte (CTL) lines. To test the efficacy of a programmable freezer (temperature falls at an optimal rate), freshly isolated lymphocytes and CTLs were each aliquoted into three sets of vials. The cells were thawed and/or washed and assayed for viability and T helper (T(sub h)0/T suppressor (T(sub s)) ratio. It is clear that omadequate freezing non-randomly damages cells of T cell subpopulations, T(sub h) being more sensitive than T(sub s). Further, it is proved that inadequate cryopreservation can confound results from a number of assessment methods owing to morphological and functional damages. The Battery of Leukocyte Tests (BLT) under development in the laboratory is designed to detect toxic, immunotoxic and genotoxic effects of in vivo mutagens exposure to human blood. |
| Supplementary Notes |
Pub. in Mutation Research, v252 n1 p1-15 Feb 91. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Non-Random Cell Killing in Cryopreservation: Implications for Performance of the Battery of Leukocyte Tests (BLT), 1. Toxic and Immunotoxic Effects. |
| Category Codes |
95B; 57F; 57Y |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
122130853 |
| Origin |
NTIS |
| Type |
CAT |
