| Main Title |
Improved Sample Recovery in Thermocycle Sequencing Protocols. |
| Author |
Ross, J. ;
Leavitt, S. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. |
| Publisher |
c1991 |
| Year Published |
1991 |
| Report Number |
EPA/600/J-91/303; |
| Stock Number |
PB92-124593 |
| Additional Subjects |
Heat ;
Base sequence ;
Deoxyribonucleic acids ;
DNA polymerases ;
Mineral oil ;
Surface tension ;
Silicones ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB92-124593 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
3p |
| Abstract |
DNA sequencing protocols which utilize multiple cycles of DNA synthesis by Taq DNA polymerase require recovery of small sample volumes from beneath a layer of mineral oil. However, the small volume of aqueous phase and the high surface tension between the two phases makes complete recovery of the aqueous phase without contaminating mineral oil difficult. These difficulties can be largely eliminated by substituting low density silicone oil for the mineral oil. The surface tension of silicone oil is lower than that of mineral oil, allowing easier recovery of the aqueous phase without contaminating oil. Also, the silicone oil is somewhat soluble in isopropanol, allowing easy removal of any trace amounts of silicone oil which may be carried over. |
| Supplementary Notes |
Pub. in Biotechniques, Benchmarks Section, v11 n5 Nov 91. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Improved Sample Recovery in Thermocycle Sequencing Protocols. |
| Category Codes |
57F |
| NTIS Prices |
PC A01/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
203826592 |
| Origin |
NTIS |
| Type |
CAT |
