| Main Title |
Characterization of Sulfhydryl Proteins Involved in the Maintenance of Flagellar Straightness in Hamster Spermatozoa. |
| Author |
Cornwall, G. A. ;
Chang., T. S. K. ;
|
| CORP Author |
Johns Hopkins Hospital, Baltimore, MD.;Health Effects Research Lab., Research Triangle Park, NC.;National Institutes of Health, Bethesda, MD. |
| Publisher |
c1990 |
| Year Published |
1990 |
| Report Number |
EPA-R-812765 ;PHS-HD-22761; EPA/600/J-90/239; |
| Stock Number |
PB91-116996 |
| Additional Subjects |
Proteins ;
Hamsters ;
Molecular weight ;
Reprints ;
Sulfhydryl compounds ;
Sperm tail ;
Sperm motility ;
Dithiothreitol ;
Two-dimensional polyacrylamide gel electrophoresis ;
Flow cytometry
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB91-116996 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
16p |
| Abstract |
Hamster caput epididymal spermatozoa exhibit a marked 90-180 degree bend when induced to acquire progressive motility in vitro (Cornwall et al, 1988). Flagellar bending is prevented by oxidizing sperm sulfhydryl (SH) groups with diamide. In the study, the authors examined the SH proteins involved in sperm flagellar bending using two-dimensional polyacrylamide gel electrophoresis and monobromobimane, an SH-specific fluorescent dye. Proteins extracted from samples containing bent caput spermatozoa contained more reduced SH than the same proteins extracted from straight caput spermatozoa. To further characterize these sperm SH proteins, caudal epididymal spermatozoa that exhibited straight flagella were induced to undergo flagellar bending by treatment with the SH reductant dithiothreitol. |
| Supplementary Notes |
Pub. in Jnl. of Andrology, v11 n2 p168-181 Mar/Apr 90. Sponsored by Health Effects Research Lab., Research Triangle Park, NC., and National Institutes of Health, Bethesda, MD. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Characterization of Sulfhydryl Proteins Involved in the Maintenance of Flagellar Straightness in Hamster Spermatozoa. |
| Category Codes |
57F |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
101123801 |
| Origin |
NTIS |
| Type |
CAT |
