| Main Title |
Metabolism of Benzo(a)Pyrene and Persistence of DNA Adducts in the Brown Bullhead 'Ictalurus nebulosus'. |
| Author |
Sikka, H. C. ;
Steward, A. R. ;
Kandaswami, C. ;
Rutkowski, J. P. ;
Zaleski, J. ;
|
| CORP Author |
State Univ. of New York Coll. at Buffalo. Div. of Environmental Toxicology and Chemistry.;Health Effects Research Lab., Research Triangle Park, NC.;Kentucky Univ., Lexington. Medical Center.;Rutgers - The State Univ., Piscataway, NJ. Dept. of Pharmacology and Toxicology. |
| Publisher |
c1991 |
| Year Published |
1991 |
| Report Number |
EPA-R-813799; EPA/600/J-92/200; |
| Stock Number |
PB92-189067 |
| Additional Subjects |
Benzo(a)pyrene ;
Metabolism ;
DNA adducts ;
Toxicology ;
Liver microsomes ;
Thin layer chromatography ;
Stereochemistry ;
Glutathione ;
Glucuronates ;
Reprints ;
Brown bullhead ;
Ictalurus nebulosus ;
Benzo(a)pyrene dihydrodiol
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB92-189067 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
6p |
| Abstract |
The in vitro metabolism of ((3)H)benzo(a)pyrene (BP) and ((14)C)benzo(a)pyrene-7,8-dihydrodiol (BP-7,8-diol) by liver of brown bullhead (Ictalurus nebulosus) was characterized, as was the formation and persistence of BP-DNA adducts in vivo. Compared to rat liver microsomes, bullhead liver microsomes produced relatively larger amounts of BP-7,8-diol (predominantly the (-) enantiomer) and smaller amounts of BP-4,5-diol. BP phase I metabolites were efficiently converted by freshly isolated bullhead hepatocytes to conjugates, predominantly glucuronides. BP-7,8-diol was metabolized by hepatocytes 4-fold more rapidly than was BP and was converted to approximately equal amounts of glucuronides, glutathione conjugates and sulfates. BP-DNA adducts formed in bullhead liver with a lag time of several days and maximum adduct formation at 25-30 days. The major adduct was anti-BPDE-deoxyguanosine. (Copyright (c) 1991 Pergamon Press.) |
| Supplementary Notes |
Pub. in Jnl. Comp. Biochem. Physiol., v100C n1/2 p25-28 1991. Sponsored by Health Effects Research Lab., Research Triangle Park, NC., Kentucky Univ., Lexington. Medical Center, and Rutgers - The State Univ., Piscataway, NJ. Dept. of Pharmacology and Toxicology. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Metabolism of Benzo(a)Pyrene and Persistence of DNA Adducts in the Brown Bullhead 'Ictalurus nebulosus'. |
| Category Codes |
57Y; 57B; 57F; 68D |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
220521610 |
| Origin |
NTIS |
| Type |
CAT |
