Main Title |
Developmental Toxicity of Methanol in Whole Embryo Culture: A Comparative Study with Mouse and Rat Embryos. |
Author |
Andrews, J. E. ;
Ebron-McCoy, M. ;
Logsdon, T. R. ;
Mole, L. M. ;
Kavlock, R. J. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Developmental Toxicology Div. ;ManTech Environmental Technology, Inc., Research Triangle Park, NC. |
Publisher |
1993 |
Year Published |
1993 |
Report Number |
EPA/600/J-94/029; |
Stock Number |
PB94-137254 |
Additional Subjects |
Methanol ;
Teratogens ;
Embryos ;
Comparison ;
Rats ;
Mice ;
Species diversity ;
Fetal development ;
Reprints ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB94-137254 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
12p |
Abstract |
Methanol (MeOH), a widely used industrial solvent, has been proposed as an alternative motor vehicle fuel. Inhaled MeOH is developmentally toxic in both rats and nice but the mouse is more sensitive than is the rat. The contribution of the embryo to this differential sensitivity was studied in whole embryo culture (WEC) using equivalent stage rat (day 9) and mouse (day 8) embryos (plug day = day 0). Rat embryos were explanted and cultured in 0, 2, 4, 8, 12 or 16 mg MeOH/ml rat serum for 24 h and then transferred to rat serum alone for 24 h. Embryonic development of the 2 and 4 mg MeOH/ml groups was not significantly different from the controls whereas the higher concentrations resulted in a concentration related decrease in somite number, head length and developmental score. The 12 mg/ml dose resulted in some embryolethality as well as dysmorphogenesis, while the highest dose was embryolethal. MeOH was dysmorphogenic in vitro in rat embryos at a MeOH concentration comparable to that reported in maternal serum following teratogenic in vivo exposures. Day 8 mouse embryos were explanted and cultured in 0, 2, 4, 6 or 8 mg MeOH/ml culture medium (75% rat serum, 25% Tyrode's salt solution) for 24 h. Embryonic development in the 2 mg/ml MeOH group was not significantly different from the controls but all higher concentration groups had a significant decrease in developmental score and crown-rump length. The high concentration group also suffered 80% embryolethality. (Copyright (c) 1993 Elsevier Scientific Publishers Ireland Ltd.) |
Supplementary Notes |
Pub. in Toxicology, v81 n3 p205-215, 27 Aug 93. Prepared in cooperation with ManTech Environmental Technology, Inc., Research Triangle Park, NC. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Developmental Toxicity of Methanol in Whole Embryo Culture: A Comparative Study with Mouse and Rat Embryos. |
Category Codes |
57Y; 57S |
NTIS Prices |
PC A03/MF A01 |
Primary Description |
600/10 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
407720042 |
Origin |
NTIS |
Type |
CAT |